Ye Meng, Liu Chuande, Li Nana, Yuan Chenhong, Liu Miaomiao, Xin Zhaojun, Lei Shu, Sun Xiaoling
Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.
Hortic Res. 2023 Sep 1;10(10):uhad178. doi: 10.1093/hr/uhad178. eCollection 2023 Oct.
Protease inhibitors promote herbivore resistance in diverse plant species. Although many inducible protease inhibitors have been identified, there are limited reports available on the biological relevance and molecular basis of constitutive protease inhibitors in herbivore resistance. Here, we identified a serine protease inhibitor, , from the tea plant (). Expression of was not strongly affected by the assessed biotic and abiotic stresses. and experiments showed that CsSERPIN1 strongly inhibited the activities of digestive protease activities of trypsin and chymotrypsin. Transient or heterologous expression of significantly reduced herbivory by two destructive herbivores, the tea geometrid and fall armyworm, in tea and plants, respectively. The expression of in did not negatively influence the growth of the plants under the measured parameters. Our findings suggest that can inactivate gut digestive proteases and suppress the growth and development of herbivores, making it a promising candidate for pest prevention in agriculture.
蛋白酶抑制剂可促进多种植物对草食动物产生抗性。尽管已鉴定出许多可诱导的蛋白酶抑制剂，但关于组成型蛋白酶抑制剂在草食动物抗性中的生物学相关性和分子基础的报道却很有限。在此，我们从茶树（）中鉴定出一种丝氨酸蛋白酶抑制剂，。的表达并未受到所评估的生物和非生物胁迫的强烈影响。和实验表明，CsSERPIN1强烈抑制胰蛋白酶和胰凝乳蛋白酶的消化蛋白酶活性。的瞬时或异源表达分别显著降低了茶尺蠖和草地贪夜蛾这两种破坏性草食动物对茶树和植物的取食。在测量参数下，在中的表达并未对植物生长产生负面影响。我们的研究结果表明，可使肠道消化蛋白酶失活并抑制草食动物的生长发育，使其成为农业害虫防治的一个有前景的候选物。
