Ellis Jeremy R, Rowley Paul A
University of Idaho, Department of Biological Sciences, Moscow, ID 83844, USA.
Johns Hopkins University, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
bioRxiv. 2023 Oct 5:2023.10.05.561034. doi: 10.1101/2023.10.05.561034.
The use of enzymes represents an approach to combat bacterial infections by degrading extracellular biomolecules to disperse biofilms. Commercial enzyme preparations, including cellulase, amylase, pectinase, zymolyase, and pepsin, exhibit concentration-dependent dispersion of biofilms. Here, we report that low concentrations of these enzymes generally lack synergy when combined or added together sequentially to biofilms. Only the addition of a protease (pepsin) followed by a commercial mixture of degradative enzymes from (zymolyase 20T), demonstrated synergy and was effective at dispersing biofilms. A more purified mixture of enzymes (zymolyase 100T) showed improved dispersal of biofilms compared to zymolyase 20T but lacked synergy with pepsin. This study emphasizes the complexity of enzymatic biofilm dispersal and the need for tailored approaches based on the properties of degradative enzymes and biofilm composition.
使用酶是一种通过降解细胞外生物分子来分散生物膜从而对抗细菌感染的方法。商业酶制剂，包括纤维素酶、淀粉酶、果胶酶、酵母裂解酶和胃蛋白酶，表现出生物膜浓度依赖性分散。在此，我们报告，当这些酶以组合或顺序添加到生物膜中时，低浓度通常缺乏协同作用。只有先添加蛋白酶（胃蛋白酶），然后添加来自酵母的商业降解酶混合物（酵母裂解酶20T），才表现出协同作用并能有效分散生物膜。与酵母裂解酶20T相比，更纯化的酶混合物（酵母裂解酶100T）显示出更好的生物膜分散效果，但与胃蛋白酶缺乏协同作用。本研究强调了酶促生物膜分散的复杂性以及基于降解酶特性和生物膜组成采取定制方法的必要性。
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