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针对生物膜的降解酶之间明显缺乏协同作用。

An apparent lack of synergy between degradative enzymes against biofilms.

作者信息

Ellis Jeremy R, Rowley Paul A

机构信息

Department of Biological Sciences, University of Idaho, Moscow, Idaho, United States.

The Johns Hopkins University School of Medicine, Johns Hopkins University, Baltimore, Maryland, United States.

出版信息

MicroPubl Biol. 2024 Mar 25;2024. doi: 10.17912/micropub.biology.001119. eCollection 2024.

DOI:10.17912/micropub.biology.001119
PMID:38596361
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11002645/
Abstract

Enzymes combat bacterial infections by degrading biomolecules to disperse biofilms. Commercial enzyme mixtures, like cellulase and pepsin, show concentration-dependent dispersion, but low concentrations lack synergy. Only the sequential addition of pepsin followed by zymolyase 20T displays synergy, effectively dispersing biofilms. Purified zymolyase 100T outperforms zymolyase 20T but lacks synergy with pepsin. This study underscores the complexity of enzymatic biofilm dispersal, highlighting the need for tailored approaches based on enzyme properties and biofilm composition.

摘要

酶通过降解生物分子来分散生物膜，从而对抗细菌感染。商业酶混合物，如纤维素酶和胃蛋白酶，表现出浓度依赖性分散，但低浓度时缺乏协同作用。只有先添加胃蛋白酶，然后再添加酵母裂解酶20T才能显示出协同作用，有效地分散生物膜。纯化的酵母裂解酶100T比酵母裂解酶20T表现更优，但与胃蛋白酶缺乏协同作用。这项研究强调了酶促生物膜分散的复杂性，突出了基于酶特性和生物膜组成采取定制方法的必要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d842/11002645/399bf519fb66/25789430-2024-micropub.biology.001119.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d842/11002645/399bf519fb66/25789430-2024-micropub.biology.001119.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d842/11002645/399bf519fb66/25789430-2024-micropub.biology.001119.jpg

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Arch Microbiol. 2022 Apr 5;204(5):243. doi: 10.1007/s00203-022-02847-4.
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