构巢曲霉中乙酰胺酶结构基因及其控制区域的精细结构图谱
Fine-structure mapping of the acetamidase structural gene and its controlling region in Aspergillus nidulans.
作者信息
Hynes M J
出版信息
Genetics. 1979 Mar;91(3):381-92. doi: 10.1093/genetics/91.3.381.
Abstract
A large number of amdS mutants altered in acetamide utilization have been used to construct a fine-structure map of the amdS locus. The mutagen diepoxyoctane generated most of the deletion strains used for mapping. A minimum of 14 sites within the amdS gene were found. Biochemical analysis of amdS mutants defined the extent of the probable coding region. A new mutant, amd-205, which did not produce detectable inactive gene product, was found to be inseparable by recombination from the "up-promoter" mutation amdI18 and was located outside of the apparent amdS coding region. The cis-dominant mutation, amdI9, was also located at this end of the gene. This work, therefore, provides evidence for the separation of a eukaryotic gene into controlling and structural regions.
摘要
大量在乙酰胺利用方面发生改变的amdS突变体已被用于构建amdS基因座的精细结构图谱。诱变剂双环氧辛烷产生了用于作图的大多数缺失菌株。在amdS基因内至少发现了14个位点。对amdS突变体的生化分析确定了可能的编码区域范围。发现一个新的突变体amd - 205，它不产生可检测到的无活性基因产物，通过重组与“上游启动子”突变amdI18无法分离，并且位于明显的amdS编码区域之外。顺式显性突变amdI9也位于该基因的这一端。因此，这项工作为将真核基因分离为控制区和结构区提供了证据。
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