Hynes M J
J Bacteriol. 1972 Sep;111(3):717-22. doi: 10.1128/jb.111.3.717-722.1972.
Aspergillus nidulans produces acetamidase and formamidase enzymes. The acetamidase is produced in reduced amounts during growth on glucose, whereas the formamidase is not greatly affected. Mutations in a gene, amdT, which affect glucose repression of amidases are described. One of these, amdT102, causes the acetamidase to be no longer subject to glucose repression and also affects ability to synthesize formamidase. The other, amdT19, results in both the formamidase and the acetamidase being subject to abnormally strong glucose repression, and also in increased maximal acetamidase activities. The dominance relationships at the amdT locus have been investigated. It is suggested that the amdT gene may play a positive role in controlling amidase synthesis.
构巢曲霉可产生乙酰胺酶和甲酰胺酶。在以葡萄糖为碳源生长时，乙酰胺酶的产量会降低，而甲酰胺酶受影响不大。本文描述了一个影响酰胺酶葡萄糖阻遏作用的基因amdT的突变情况。其中一个突变体amdT102，导致乙酰胺酶不再受葡萄糖阻遏，同时也影响甲酰胺酶的合成能力。另一个突变体amdT19，则导致甲酰胺酶和乙酰胺酶都受到异常强烈的葡萄糖阻遏，并且乙酰胺酶的最大活性增加。研究了amdT位点的显性关系。结果表明，amdT基因可能在控制酰胺酶合成中起正向作用。
