Yas Baydaa Ahmed
Department of Paedodontic, Orthodontics and Preventive Dentistry, College of Dentistry, Uruk University, Baghdad, Iraq.
J Int Soc Prev Community Dent. 2023 Aug 30;13(4):318-326. doi: 10.4103/jispcd.JISPCD_186_22. eCollection 2023 Jul-Aug.
Oxidant and antioxidant components in saliva play an essential role in caries development. The purpose of the current study was to disclose the interactive effect of salivary protein carbonyl (PC), total glutathione (GSH), pH, and flow rate on root caries severity.
The control and study groups consisted of 90 older adults of both genders classified into six groups: normal salivary flow rate with no root caries (control), normal salivary flow rate with incipient root caries, normal salivary flow rate with shallow root caries, hyposalivation with no root caries, hyposalivation with incipient root caries, and hyposalivation with shallow root caries. Each group consisted of 15 older adults. The study participants were selected from those patients who attended the teaching hospital at the College of the Dentistry/University of Baghdad and fit the study's criteria. Unstimulated saliva was collected. Both salivary pH and flow rate were determined immediately. After that, saliva was subjected to biochemical analysis to determine PC and total GSH levels colorimetrically. Root surface caries was diagnosed clinically using the Root Caries Index. Data were statistically analyzed using descriptive statistics, two-way univariate analysis of variance, two-way multivariate analysis of variance, and Pearson's correlation coefficient ( = 5%).
Salivary total glutathione revealed a significant interactive effect with salivary flow rate and root decay severity. Levels of salivary total GSH were significantly higher in subjects with shallow root caries than those with incipient root caries; no root caries levels of salivary PC were significantly high in the hyposalivation group, but no correlation with caries severity was found. High root caries severity was found to be associated with reduced salivary pH and flow rate.
A significant interactive effect was recorded for salivary flow rate, pH, and total GSH on root caries severity except for salivary PC.
唾液中的氧化剂和抗氧化剂成分在龋齿发展过程中起着至关重要的作用。本研究的目的是揭示唾液蛋白羰基（PC）、总谷胱甘肽（GSH）、pH值和流速对根龋严重程度的交互作用。
对照组和研究组由90名老年男女组成，分为六组：唾液流速正常且无根龋（对照组）、唾液流速正常且有初期根龋、唾液流速正常且有浅根龋、唾液分泌过少且无根龋、唾液分泌过少且有初期根龋、唾液分泌过少且有浅根龋。每组由15名老年人组成。研究参与者从那些到巴格达大学牙科学院教学医院就诊且符合研究标准的患者中选取。收集未刺激唾液。立即测定唾液的pH值和流速。之后，对唾液进行生化分析，通过比色法测定PC和总GSH水平。使用根龋指数对根面龋进行临床诊断。数据采用描述性统计、双向单因素方差分析、双向多因素方差分析和Pearson相关系数进行统计学分析（α = 5%）。
唾液总谷胱甘肽显示出与唾液流速和根龋严重程度有显著的交互作用。浅根龋患者的唾液总GSH水平显著高于初期根龋患者；唾液分泌过少组的唾液PC无根龋水平显著升高，但未发现与龋严重程度相关。发现高根龋严重程度与唾液pH值和流速降低有关。
除唾液PC外，唾液流速、pH值和总GSH对根龋严重程度有显著的交互作用。
