Akal Eser
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun, Türkiye.
Front Vet Sci. 2023 Oct 9;10:1283128. doi: 10.3389/fvets.2023.1283128. eCollection 2023.
Worldwide, various counting chambers and computer-assisted sperm analysis (CASA) devices are in use. The semen's concentration can vary depending on the depth of the counting chamber and how it is loaded. The study's objectives were to analyze the effects of various counting chambers on semen concentration results using a GoldCyto® slide and a glass slide in the CASA system and to ascertain the precision of concentration measurements made using glass slides on CASA. The study's control group was composed of samples with known concentrations (72-80 million sperm/mL) as determined by a spectrophotometer. A total of 21 frozen straws from the same bull of the same date were thawed at 37°C for 30 s and loaded into two different sperm-counting chambers (GoldCyto® slide and glass slide). The sample semen placed in the sperm counting chambers was 5 μL and the same value was entered in the CASA software as 5 μL. Measurements were done and evaluated in 5 different areas. According to the data we obtained, using the glass slide were statistically lower than the spectrophotometer ( < 0.001). GoldCyto® slide results were consistent with spectrophotometer results. Consequently, measurements with GoldCyto® slides in the CASA had consistent results, while measurements with glass slides were inconsistent. It was concluded that GoldCyto® slides are more suitable than glass slides in the concentration examinations of semen. Therefore, more study is needed to optimize the use of glass slides.
在全球范围内，各种血细胞计数板和计算机辅助精子分析（CASA）设备都在使用。精液浓度可能会因计数板的深度及其加载方式而有所不同。本研究的目的是分析在CASA系统中使用GoldCyto®载玻片和玻璃载玻片时，各种计数板对精液浓度结果的影响，并确定使用玻璃载玻片在CASA上进行浓度测量的精度。该研究的对照组由分光光度计测定的已知浓度（7200万-8000万精子/mL）的样本组成。将来自同一头公牛、同一日期的总共21根冷冻细管在37°C解冻30秒，然后加载到两个不同的精子计数板（GoldCyto®载玻片和玻璃载玻片）中。放入精子计数板中的精液样本为5μL，并且在CASA软件中输入相同的值5μL。在5个不同区域进行测量和评估。根据我们获得的数据，使用玻璃载玻片测得的结果在统计学上低于分光光度计（<0.001）。GoldCyto®载玻片的结果与分光光度计的结果一致。因此，在CASA中使用GoldCyto®载玻片进行测量结果一致，而使用玻璃载玻片进行测量则不一致。得出的结论是，在精液浓度检测中，GoldCyto®载玻片比玻璃载玻片更合适。因此，需要更多研究来优化玻璃载玻片的使用。
