Björndahl Lars
Andrology Laboratory, Centre for Andrology and Sexual Medicine, Clinic for Endocrinology, Department of Medicine, Huddinge, Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Methods Mol Biol. 2013;927:3-12. doi: 10.1007/978-1-62703-038-0_1.
Proper assessment of the number of spermatozoa is essential not only as an initial step in every clinical infertility investigation [Björndahl et al (2010) A practical guide to basic laboratory andrology, 1st edn. Cambridge University Press, Cambridge] but also when attempting to establish the total sperm production in the testis [Amann (Hum Reprod 25:22-28, 2010); Amann (J Androl 30:626-641, 2009); Amann and Chapman (J Androl 30:642-649, 2009)]. Reliable methods combined with an understanding of the specific physiology involved as well as the main sources of errors related to the assessment of sperm concentration are critical for ensuring accurate concentration determination [Björndahl et al (2010) A practical guide to basic laboratory andrology, 1st edn. Cambridge University Press, Cambridge; World Health Organization (2010) WHO laboratory manual for the examination and processing of human semen. WHO, Geneva]. This chapter therefore focuses on these three aspects.
正确评估精子数量至关重要，这不仅是每项临床不孕症检查的初始步骤[比约恩达尔等人（2010年）《基础男科学实用指南》，第1版。剑桥大学出版社，剑桥]，也是试图确定睾丸中精子的总生成量时所必需的[阿曼（《人类生殖》25:22 - 28，2010年）；阿曼（《雄性学杂志》30:626 - 641，2009年）；阿曼和查普曼（《雄性学杂志》30:642 - 649，2009年）]。可靠的方法，结合对相关特定生理学的理解以及与精子浓度评估相关的主要误差来源，对于确保准确的浓度测定至关重要[比约恩达尔等人（2010年）《基础男科学实用指南》，第1版。剑桥大学出版社，剑桥；世界卫生组织（2010年）《世界卫生组织人类精液检查与处理实验室手册》。世界卫生组织，日内瓦]。因此，本章重点关注这三个方面。
