Craig P S, Bailey W, Nelson G S
Trans R Soc Trop Med Hyg. 1986;80(2):256-7. doi: 10.1016/0035-9203(86)90029-5.
A simple and sensitive two-step enzyme immunoassay for Echinococcus antigen was developed, using peroxidase conjugated IgG fractions of rabbit anti-Echinococcus granulosus cyst fluid serum or of pooled sera from human hydatid patients, to assay samples of cystic fluid taken from patients with suspected hydatid cyst infections. The assay was 100% positive with nine proven human hydatid cyst fluids; in six patients with cysts where serology was negative, there was no evidence of Echinococcus antigen in the cyst fluid. This simple procedure is therefore of value in confirming or excluding the diagnosis of hydatid in cystic fluid obtained by biopsy or after surgery.
开发了一种用于检测棘球蚴抗原的简单且灵敏的两步酶免疫测定法，该方法使用与兔抗细粒棘球蚴囊液血清或人包虫病患者混合血清的IgG级分结合的过氧化物酶，来检测疑似包虫囊肿感染患者的囊液样本。对于9份经证实的人包虫囊肿液，该测定法的阳性率为100%；在6例囊肿血清学检测为阴性的患者中，囊液中未发现棘球蚴抗原。因此，这种简单的方法对于通过活检或手术后获得的囊液中包虫病的诊断确认或排除具有重要价值。
