Ravinder P T, Parija S C
Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India.
Acta Trop. 1997 Sep 10;66(3):169-73. doi: 10.1016/s0001-706x(97)00036-3.
The exact aetiology of a suspected hydatid cyst may sometimes be a diagnostic dilemma. One of the recent methods to confirm that the cysts are echinococcal in origin is the demonstration of hydatid antigens in the aspirated cystic fluid. In the present study, we evaluated the use of countercurrent immunoelectrophoresis (CIEP) to detect hydatid antigen in cyst fluids. Antibody used for detecting the antigen consisted of hyper immune sera raised in rabbits after inoculation of crude human hydatid cystic fluid antigen. Fluids were collected post-operatively from a total of 14 hydatid cysts confirmed by surgery and by histopathology. The results of the study show that the test is moderately sensitive detecting antigen in 11 (78.5%) of 14 cyst fluids. The test did not detect antigen in three other cyst fluids. The main advantage is its specificity as it was 100% specific with no reactions in control samples. Even though, sensitivity is not very high, the test is sample, inexpensive, and can rapidly diagnose hydatid aetiology of cysts and may be of help in the diagnosis of hydatid cysts in peripheral parasitology laboratories.
疑似包虫囊肿的确切病因有时可能是一个诊断难题。确认囊肿起源于棘球蚴的最新方法之一是在吸出的囊液中检测包虫抗原。在本研究中，我们评估了逆流免疫电泳（CIEP）在检测囊液中包虫抗原的应用。用于检测抗原的抗体由接种粗制人包虫囊液抗原后在兔体内产生的超免疫血清组成。术后从总共14个经手术和组织病理学证实的包虫囊肿中收集囊液。研究结果表明，该检测方法具有中等敏感性，在14份囊液中的11份（78.5%）检测到了抗原。在其他3份囊液中未检测到抗原。其主要优点是特异性，因为它具有100%的特异性，对照样本中无反应。尽管敏感性不是很高，但该检测方法简单、廉价，能够快速诊断囊肿的包虫病因，可能有助于外周寄生虫学实验室诊断包虫囊肿。
