Herbarium and Pharmacognosy, School of Chemistry, Biochemistry, and Pharmacy, National University of San Luis, San Luis, Argentina.
Pharmacognosy, School of Biochemical and Pharmaceutical Sciences, National University of Rosario, Rosario, Argentina.
Photochem Photobiol. 2024 May-Jun;100(3):686-698. doi: 10.1111/php.13871. Epub 2023 Oct 25.
Despite significant progress in the development of phototherapy drugs, it is widely recognized that natural products remain the primary source of new photoactive compounds. Exploring uncharted flora in the east-central region of Argentina may offer a vast array of opportunities to isolate new photoactive molecules or plant extracts with high potential for use in antimicrobial photodynamic therapy (aPDT) against Candida albicans. To assess the photofungicidal potential of T. sinuata ("contrayerba") against C. albicans, the extracts underwent spectroscopic and chromatographic analysis, resulting in the identification of furanocoumarin metabolites with similar spectrophotometric properties in all extracts. The extract profiles were created using UHPLC-DAD, and seven furanocoumarins (FCs) were detected. The highest photoinactivation against C. albicans was observed for dicholormethanic extracts (MFC = 62.5 μg/mL), equal to xanthotoxin employed as a positive control. Furthermore, we determine that photochemical mechanisms dependent on oxygen (type I and type II processes) and mechanisms independent of oxygen (photoadduct formation) are involved in the death of these yeasts. These results support the use of native plants of the east-central region of Argentina as potent sensitizers for aPDT and suggest that they can replace xanthotoxin in treating superficial yeast infections of the skin.
尽管光疗药物的发展取得了重大进展，但人们普遍认为天然产物仍然是新的光活性化合物的主要来源。探索阿根廷中东部未开发的植物群，可能有机会分离出新的光活性分子或具有很大潜力的植物提取物，用于抗白色念珠菌的光动力抗菌治疗（aPDT）。为了评估 T. sinuata（“contrayerba”）对 C. albicans 的光杀菌潜力，提取物进行了光谱和色谱分析，结果在所有提取物中鉴定出具有相似分光光度特性的呋喃并香豆素代谢物。使用 UHPLC-DAD 创建了提取物图谱，并检测到七种呋喃并香豆素（FC）。对 C. albicans 的最高光失活作用是二氯甲烷提取物（MFC = 62.5μg/mL），与用作阳性对照的黄曲毒素相当。此外，我们确定依赖于氧的光化学机制（I 型和 II 型过程）和不依赖于氧的机制（光加合物形成）参与了这些酵母的死亡。这些结果支持将阿根廷中东部地区的本地植物用作有效的 aPDT 敏化剂，并表明它们可以替代黄曲毒素来治疗皮肤浅表真菌感染。
