Department of Proteomics, Discovery Life Sciences, Huntsville, Alabama 35806, United States.
Department of Environmental Health Sciences, Yale School of Public Health, Yale University, New Haven, Connecticut 06520, United States.
J Proteome Res. 2023 Nov 3;22(11):3401-3417. doi: 10.1021/acs.jproteome.3c00167. Epub 2023 Oct 25.
Imaging mass spectrometry is a well-established technology that can easily and succinctly communicate the spatial localization of molecules within samples. This review communicates the recent advances in the field, with a specific focus on matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) applied on tissues. The general sample preparation strategies for different analyte classes are explored, including special considerations for sample types (fresh frozen or formalin-fixed,) strategies for various analytes (lipids, metabolites, proteins, peptides, and glycans) and how multimodal imaging strategies can leverage the strengths of each approach is mentioned. This work explores appropriate experimental design approaches and standardization of processes needed for successful studies, as well as the various data analysis platforms available to analyze data and their strengths. The review concludes with applications of imaging mass spectrometry in various fields, with a focus on medical research, and some examples from plant biology and microbe metabolism are mentioned, to illustrate the breadth and depth of MALDI IMS.
成像质谱是一种成熟的技术，能够简洁地传达样品中分子的空间定位。本综述介绍了该领域的最新进展，特别关注应用于组织的基质辅助激光解吸/电离（MALDI）成像质谱（IMS）。探讨了不同分析物类别的一般样品制备策略，包括对特殊样本类型（新鲜冷冻或福尔马林固定）的考虑、各种分析物（脂质、代谢物、蛋白质、肽和聚糖）的策略以及如何利用每种方法的优势的多模态成像策略。这项工作探讨了成功研究所需的合适实验设计方法和流程标准化，以及可用于分析数据及其优势的各种数据分析平台。综述最后介绍了成像质谱在各个领域的应用，重点是医学研究，并提到了植物生物学和微生物代谢方面的一些例子，以说明 MALDI IMS 的广度和深度。
