FTO基因分型（rs9939609 T>A）与健康年轻成年人添加糖摄入量增加有关。

FTO genotypes (rs9939609 T>A) are Associated with Increased Added Sugar Intake in Healthy Young Adults.

作者信息

Madrigal-Juarez Andrea, Martínez-López Erika, Sanchez-Murguia Tania, Magaña-de la Vega Lisset, Rodriguez-Echevarria Roberto, Sepulveda-Villegas Maricruz, Torres-Valadez Rafael, Torres-Castillo Nathaly

机构信息

Instituto de Nutrigenética y Nutrigenómica Traslacional, Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Mexico.

Doctorado en Ciencias de la Nutrición Traslacional, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Mexico.

出版信息

Lifestyle Genom. 2023;16(1):214-223. doi: 10.1159/000534741. Epub 2023 Oct 25.

DOI:10.1159/000534741

PMID:37879306

Abstract

INTRODUCTION

Single-nucleotide polymorphism (SNP) rs9939609 in the FTO gene has been associated with dietary intake and appetite traits, mainly in participants with obesity; however, it remains widely unexplored in normal weight participants. Thus, the aims of this study were (1) to compare the changes in subjective appetite sensations, ghrelin, and insulin concentrations according to the SNP rs9939609 T>A in FTO and (2) to compare dietary intake between rs9939609 genotype groups in normal weight young participants.

METHODS

We conducted a quasi-experimental study involving 88 normal weight participants to analyze subjective perception of appetite, hormonal response for hunger and satiety, and dietary intake according to the rs9939609 SNP. Participants received a standardized single breakfast. Visual analogue scales (VAS) were utilized for assessing the subjective perception of appetite at fasting and immediately after breakfast and at 30, 60, 90, and 120 min postprandially. Glucose, lipid profile, ghrelin, and insulin were measured at fasting and at 120 min after breakfast. Dietary intake was assessed with a 3-day food record. The SNP was determined by allelic discrimination with TaqMan probes. To compare dietetic, biochemical, and the subjective appetite sensations, Student t test, ANCOVA test, and the repeated measures ANOVA were used. The linear regression model and the linear mixed model were used for the association analysis. Pearson correlation was used to test the correlation between two quantitative variables.

RESULTS

A total of 88 people participated, 81.8% were female, with a mean body mass index of 21.8 ± 2.0 kg/m2 and a mean age of 20.6 ± 2.0. Genotype frequencies of the rs9939609 SNP were 52% for the TT allele and 48% for the TA/AA. The subjective perception of appetite named hunger, fullness, satiety, desire to eat, and prospective food consumption were similar between genotypes of the rs9939609. Participants with the TA/AA genotype showed a higher intake of added sugar (p = 0.039) than TT participants. No differences were found in ghrelin, insulin, glucose, or lipid parameters between genotypes.

CONCLUSION

Carriers of the A allele from FTO gene SNP rs9939609 may have an increased preference for foods, specifically for added sugars.

摘要

引言

FTO基因中的单核苷酸多态性（SNP）rs9939609与饮食摄入和食欲特征有关，主要见于肥胖参与者；然而，在正常体重参与者中仍未得到广泛研究。因此，本研究的目的是：（1）根据FTO基因中SNP rs9939609的T>A比较主观食欲感受、胃饥饿素和胰岛素浓度的变化；（2）比较正常体重年轻参与者中rs9939609基因型组之间的饮食摄入量。

方法

我们进行了一项准实验研究，纳入88名正常体重参与者，根据rs9939609 SNP分析食欲的主观感知、饥饿和饱腹感的激素反应以及饮食摄入量。参与者接受标准化的单一早餐。使用视觉模拟量表（VAS）评估空腹时、早餐后即刻以及餐后30、60、90和120分钟时的食欲主观感知。在空腹时和早餐后120分钟测量血糖、血脂、胃饥饿素和胰岛素。通过3天的饮食记录评估饮食摄入量。使用TaqMan探针通过等位基因鉴别确定SNP。为比较饮食、生化和主观食欲感受，使用了学生t检验、协方差分析（ANCOVA）和重复测量方差分析。线性回归模型和线性混合模型用于关联分析。Pearson相关性用于检验两个定量变量之间的相关性。

结果

共有88人参与，81.8%为女性，平均体重指数为21.8±2.0kg/m²，平均年龄为20.6±2.0。rs9939609 SNP的基因型频率为TT等位基因52%，TA/AA为48%。rs9939609不同基因型之间，饥饿、饱腹感、饱足感、进食欲望和预期食物摄入量等食欲主观感知相似。TA/AA基因型参与者的添加糖摄入量高于TT基因型参与者（p = 0.039）。不同基因型之间在胃饥饿素、胰岛素、血糖或血脂参数方面未发现差异。