Frontier Science Centre for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), Collaborative Innovation Centre of Chemical Science and Engineering (Tianjin), and School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, PR China.
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China.
Biotechnol Adv. 2022 Jan-Feb;54:107808. doi: 10.1016/j.biotechadv.2021.107808. Epub 2021 Jul 27.
Enzymes catalyse target reactions under mild conditions with high efficiency, as well as excellent regional-, stereo-, and enantiomeric selectivity. Photocatalysis utilises sustainable and environment-friendly light power to realise efficient chemical conversion. By combining the interdisciplinary advantages of photo- and enzymatic catalysis, the photocatalyst-enzyme hybrid systems have proceeded various light-driven biotransformation with high efficiency under environmentally benign conditions, thus, attracting unparalleled focus during the last decades. It has also been regarded as a promising pathway towards green chemistry utilising ubiquitous solar energy. This systematic review gives insight into this research field by classifying the existing photocatalyst-enzyme hybrid systems into three sections based on different hybridizing modes between photo- and enzymatic catalysis. Furthermore, existing challenges and proposed strategies are discussed within this context. The first system summarised is the cofactor-mediated hybrid system, in which natural/artificial cofactors act as reducing equivalents that connect photocatalysts with enzymes for light-driven enzymatic biotransformation. Second, the direct contact-based photocatalyst-enzyme hybrid systems are described, including two different kinds of electron exchange sites on the enzyme molecules. Third, some cases where photocatalysts and enzymes are integrated into a reaction cascade with specific intermediates will be discussed in the following chapter. Finally, we provide perspective concerning the future of this field.
酶在温和条件下以高效率催化目标反应，同时具有出色的区域、立体和对映选择性。光催化利用可持续且环保的光能实现高效的化学转化。通过结合光催化和酶催化的跨学科优势，光催化剂-酶杂化系统在环境友好的条件下以高效率进行了各种光驱动的生物转化，因此在过去几十年中引起了无与伦比的关注。它也被认为是利用普遍存在的太阳能实现绿色化学的有前途的途径。本系统评价通过根据光催化和酶催化之间不同的杂交模式将现有的光催化剂-酶杂化系统分为三个部分，深入了解该研究领域。此外，还在这一背景下讨论了现有的挑战和提出的策略。总结的第一个系统是辅因子介导的杂化系统，其中天然/人工辅因子作为还原当量，将光催化剂与酶连接起来，用于光驱动的酶生物转化。其次，描述了基于直接接触的光催化剂-酶杂化系统，包括酶分子上的两种不同的电子交换位点。第三，将在以下章节中讨论将光催化剂和酶整合到具有特定中间体的反应级联中的一些情况。最后，我们对该领域的未来提供了一些看法。
