Department of Orthopaedics, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, 226001, China.
Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, 226001, China.
J Orthop Surg Res. 2023 Oct 25;18(1):800. doi: 10.1186/s13018-023-04273-8.
Osteoarthritis (OA) is a degenerative disease. It is common in middle-aged and elderly people and is one of the main causes of disability. Currently, the etiology of OA is unclear, and no specific biomarkers for the diagnosis of OA have been identified. Therefore, finding a highly sensitive biomarker is essential for a proper diagnosis.TRNA-derived fragments (tRFs) and tRNA-derived stress-induced RNAs (tiRNAs) are newly discovered classes of noncoding RNAs. tRF has been proven in several studies to have significant associations with tumor diagnosis, making it a promising biomarker in cancer research. However, the diagnostic utility of tRF in OA patients and the correlation between OA progression and trf differential expression have yet to be elaborated. The purpose of this research was to identify tRFs with differential expression in OA to assess their potential as OA biomarkers. To determine the tRF-5022B expression level in this research, real-time fluorescence quantitative PCR has been employed. Agarose gel electrophoresis, Sanger sequencing, and other investigations have been employed for evaluating tRF-5022B's molecular properties. Receiver operating characteristic curve analysis has been utilized for assessing the diagnostic effectiveness of the tRF-5022B. The findings demonstrated that tRF-5022B expression was considerably lower in OA serum. The Kellgren-Lawrence grading scale was shown to correspond with serum expression levels. The ROC curve confirmed that tRF-5022B serum expression levels might differentiate OA cases from healthy individuals and RA patients. According to the aforementioned findings, tRF-5022B may be employed as a novel biomarker for OA diagnosis due to its excellent diagnostic value.
骨关节炎（OA）是一种退行性疾病。它在中老年人中很常见，是导致残疾的主要原因之一。目前，OA 的病因尚不清楚，也没有发现用于 OA 诊断的特定生物标志物。因此，寻找高度敏感的生物标志物对于正确诊断至关重要。tRNA 衍生片段（tRFs）和 tRNA 衍生应激诱导 RNA（tiRNAs）是新发现的一类非编码 RNA。几项研究已经证明 tRF 与肿瘤诊断有显著关联，使其成为癌症研究中有前途的生物标志物。然而，tRF 在 OA 患者中的诊断效用以及 OA 进展与 trf 差异表达之间的相关性尚未得到详细阐述。本研究的目的是鉴定 OA 中差异表达的 tRFs，以评估其作为 OA 生物标志物的潜力。为了确定本研究中 tRF-5022B 的表达水平，采用了实时荧光定量 PCR。采用琼脂糖凝胶电泳、Sanger 测序等方法对 tRF-5022B 的分子特性进行了评估。采用受试者工作特征曲线分析评估了 tRF-5022B 的诊断效果。研究结果表明，OA 血清中 tRF-5022B 的表达明显降低。Kellgren-Lawrence 分级量表与血清表达水平相对应。ROC 曲线证实，tRF-5022B 血清表达水平可区分 OA 病例与健康个体和 RA 患者。根据上述发现，tRF-5022B 可能因其出色的诊断价值而被用作 OA 诊断的新型生物标志物。
