血清转移 RNA 衍生片段 tRF-31-79MP9P9NH57SD 可作为非小细胞肺癌的新型诊断生物标志物。
Serum transfer RNA-derived fragment tRF-31-79MP9P9NH57SD acts as a novel diagnostic biomarker for non-small cell lung cancer.
机构信息
Department of Central Laboratory, The Affiliated People's Hospital, Ningbo University, Ningbo, China.
Department of Respiratory and Critical Medicine, Ningbo First Hospital, Ningbo, China.
出版信息
J Clin Lab Anal. 2022 Jul;36(7). doi: 10.1002/jcla.24492. Epub 2022 May 16.
BACKGROUND
tRNA-derived fragments (tRFs) have been found to have a crucial function in the pathophysiology of cancers. However, the function of tRFs in non-small cell lung cancer (NSCLC) is yet unknown. The goal of this study was to assess the tRF-31-79MP9P9NH57SD serum expression from NSCLC patients and to determine its diagnostic usefulness.
METHODS
By using stem-loop quantitative real-time PCR, we were able to detect various tRF-31-79MP9P9NH57SD expressions in 96 NSCLC serum samples, 96 healthy controls, and 20 pairs of NSCLC serum samples pre- and post-surgery (qRT-PCR). After that, we analyzed its diagnostic effectiveness using the receiver operating characteristic (ROC) curve.
RESULTS
Serum tRF-31-79MP9P9NH57SD expression was higher in NSCLC patients, and levels of tRF-31-79MP9P9NH57SD were linked to the clinical stage (p = 0.002) and the malignancy of lymph node (p = 0.012). In addition, after the procedure, the serum tRF-31-79MP9P9NH57SD expression in NSCLC patients dropped. With 48.96 percent sensitivity and 90.62 percent specificity, the area under ROC curve (AUC) was 0.733.
CONCLUSION
serum tRF-31-79MP9P9NH57SD possibly is a new and groundbreaking biomarker for the NSCLC.
背景
tRNA 衍生片段（tRFs）在癌症的病理生理学中具有重要作用。然而，tRFs 在非小细胞肺癌（NSCLC）中的功能尚不清楚。本研究旨在评估 NSCLC 患者血清中的 tRF-31-79MP9P9NH57SD 表达，并确定其诊断价值。
方法
我们使用茎环定量实时 PCR 检测了 96 例 NSCLC 血清样本、96 例健康对照和 20 对 NSCLC 术前和术后血清样本中的各种 tRF-31-79MP9P9NH57SD 表达（qRT-PCR）。然后，我们使用接收者操作特征（ROC）曲线分析其诊断效果。
结果
NSCLC 患者血清 tRF-31-79MP9P9NH57SD 表达升高，tRF-31-79MP9P9NH57SD 水平与临床分期（p=0.002）和淋巴结恶性程度（p=0.012）相关。此外，手术治疗后 NSCLC 患者血清 tRF-31-79MP9P9NH57SD 表达降低。ROC 曲线下面积（AUC）为 0.733，灵敏度为 48.96%，特异性为 90.62%。
结论
血清 tRF-31-79MP9P9NH57SD 可能是 NSCLC 的一种新的突破性生物标志物。
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