School of Grassland Science, Beijing Forestry University, Beijing 100083, China.
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
J Agric Food Chem. 2023 Nov 8;71(44):16657-16668. doi: 10.1021/acs.jafc.3c03922. Epub 2023 Oct 25.
The expression of plant genes under salt stress at the transcriptional level has been extensively studied. However, less attention has been paid to gene translation regulation under salt stress. In this study, Ribo-seq and RNA-seq analyses were conducted in seedlings grown under normal and salt stress conditions. The results showed that salt stress significantly altered the gene expression at the transcriptional and translational levels, with 2755 genes showing significant changes only at the translational level. Salt stress significantly inhibited the gene translation efficiency. Small ORFs (including uORFs in the 5'UTR, dORFs in 3'UTRs, and sORFs in lncRNAs) were identified throughout the genome of . The efficiency of gene translation was simultaneously regulated by the uORFs, dORFs, and miRNAs. In summary, our results provide valuable information about translatomic resources and new insights into plant responses to salt stress.
盐胁迫下植物基因在转录水平上的表达已经得到了广泛的研究。然而，盐胁迫下基因翻译调控的关注较少。在这项研究中，对正常和盐胁迫条件下生长的 幼苗进行了 Ribo-seq 和 RNA-seq 分析。结果表明，盐胁迫显著改变了转录和翻译水平上的基因表达，有 2755 个基因仅在翻译水平上表现出显著变化。盐胁迫显著抑制了基因翻译效率。在 的整个基因组中鉴定出了小 ORFs（包括 5'UTR 中的 uORFs、3'UTRs 中的 dORFs 和 lncRNAs 中的 sORFs）。基因翻译的效率同时受到 uORFs、dORFs 和 miRNAs 的调节。总之，我们的研究结果提供了有价值的转译资源信息，并为植物对盐胁迫的响应提供了新的见解。
