Liang Wangli, Zhang Zheng, Yao Ning, Wang Bo, Yu Wenjing, Zhu Qiang, Yang Shujuan, Zeng Jijuan, Wang Lingxia, Liang Wenyu
School of Life Sciences, Ningxia University, Yinchuan, Ningxia, 750021, China.
College of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia, 750021, China.
BMC Plant Biol. 2025 Mar 31;25(1):405. doi: 10.1186/s12870-025-06402-3.
Lycium barbarum L. possesses great salt tolerance and medicinal values, studying its salt tolerance contribute to variety improvement, as well as the increase in yield and quality.
The study integrated the tandem mass tags (TMT) phosphoproteomics and physiological indexes of L. barbarum exposed to different concentrations of NaCl, with the aim of characterizing salt adaptation characteristics of L. barbarum. The findings indicated that a total of 2189 differentially phosphorylated peptides were identified, functional analysis revealed their involvement in glycolysis, plant hormone signal transduction, mitogen-activated protein kinase (MAPK) signal transduction and other pathways, and that the enzyme activities and substances related to glycolysis and signal transduction underwent significant changes under salt stress.
Salt stress enhanced the glycolysis pathway through protein phosphorylation, and the changes in related enzymes activity accelerated the conversion of intermediate metabolites and energy supply. Salt stress led to the accumulation of abscisic acid (ABA), jasmonic acid (JA), and salicylic acid (SA) levels, triggering signal transduction events regulated by phosphorylated proteins to improve salt tolerance for L. barbarum in saline environments. The phosphorylation of MAPK signaling pathway-related proteins is triggered by reactive oxygen species (ROS) and ABA as signal molecules to induce the expression of downstream salt stress response factors. This study provides a foundation for further analysis of the molecular regulatory mechanism of protein phosphorylation in L. barbarum for salt stress.
枸杞具有很强的耐盐性和药用价值，研究其耐盐性有助于品种改良以及产量和品质的提高。
本研究整合了暴露于不同浓度氯化钠的枸杞的串联质谱标签（TMT）磷酸化蛋白质组学和生理指标，旨在表征枸杞的盐适应特性。研究结果表明，共鉴定出2189个差异磷酸化肽段，功能分析显示它们参与糖酵解、植物激素信号转导、丝裂原活化蛋白激酶（MAPK）信号转导等途径，并且在盐胁迫下，与糖酵解和信号转导相关的酶活性和物质发生了显著变化。
盐胁迫通过蛋白质磷酸化增强了糖酵解途径，相关酶活性的变化加速了中间代谢产物的转化和能量供应。盐胁迫导致脱落酸（ABA）、茉莉酸（JA）和水杨酸（SA）水平积累，触发由磷酸化蛋白调节的信号转导事件，以提高枸杞在盐渍环境中的耐盐性。活性氧（ROS）和ABA作为信号分子触发MAPK信号通路相关蛋白的磷酸化，诱导下游盐胁迫响应因子的表达。本研究为进一步分析枸杞中蛋白质磷酸化对盐胁迫的分子调控机制奠定了基础。
