MacPherson B R, Yiu V, Lee W, Scott G W
Acta Anat (Basel). 1986;127(1):59-64. doi: 10.1159/000146238.
A connective tissue digestion and removal technique utilizing collagenase and hyaluronidase incubation followed by treatment with 8 N hydrochloric acid successfully revealed the muscular components within the gallbladder wall of the dog, allowing three-dimensional visualization with the scanning electron microscope. The muscle adjacent to the serosa appeared as a homogeneous layer with few discontinuities. In contrast to this were the more superficial muscle layers which were arranged as a meshwork of interlacing smooth muscle bundles of various size, the interstices of which were filled with cellular connective tissue. These gaps in the muscle layer varied in size, becoming larger as the muscle bundles tapered out just below the mucosal aspect of the gallbladder wall.
一种利用胶原酶和透明质酸酶孵育，随后用8N盐酸处理的结缔组织消化和去除技术，成功地揭示了狗胆囊壁内的肌肉成分，从而能够用扫描电子显微镜进行三维可视化观察。与浆膜相邻的肌肉呈现为一层均匀的结构，几乎没有间断。与此形成对比的是，较浅的肌肉层排列成由各种大小的交错平滑肌束组成的网状结构，其间隙充满了细胞性结缔组织。肌肉层中的这些间隙大小不一，随着肌束在胆囊壁黏膜面下方逐渐变细而变大。
