Miura K, Ishimaru Y, Yoshimura T
Acta Pathol Jpn. 1986 Sep;36(9):1419-27. doi: 10.1111/j.1440-1827.1986.tb02864.x.
A rare type of mucoepidermoid tumor of hard palate was found in a 42-year-old man. By light microscopic examination, the nests of the tumor consisted almost entirely of clear cells and partly of epidermoid cells observed at the periphery of the nests. In addition, only a few isolated mucous cells were dispersed in sheets of the clear cells. Transition between the epidermoid cells and clear cells was apparent, characterized by a progressive clearing of the cytoplasm. It was shown by both the PAS reaction and removal by diastase digestion that the clear cytoplasm is due to the presence of significant amount of glycogen granules. By electron microscopic examination, adhesiveness of the epidermoid cells and the clear cells was characterized by numerous desmosomes. There were observed bundles of cytoplasmic tonofilaments and desmosome-tonofilament attachment complexes in these cells. There was a variable amount of amorphous lucent substance in the epidermoid and clear cells, particularly in the clear cells. It was assumed that glycogen is lost from cytoplasm during en bloc staining with uranyl acetate and the matrix of the glycogen areas appears as the amorphous feature.
一名42岁男性被发现患有罕见类型的硬腭黏液表皮样肿瘤。通过光学显微镜检查，肿瘤巢几乎完全由透明细胞组成，部分表皮样细胞位于巢的周边。此外，只有少数孤立的黏液细胞分散在透明细胞片中。表皮样细胞和透明细胞之间的过渡明显，其特征是细胞质逐渐变清。PAS反应和淀粉酶消化去除实验均表明，透明细胞质是由于存在大量糖原颗粒。通过电子显微镜检查，表皮样细胞和透明细胞的黏附性表现为大量桥粒。在这些细胞中观察到成束的细胞质张力丝和桥粒-张力丝附着复合体。表皮样细胞和透明细胞中存在数量不等的无定形透明物质，尤其是在透明细胞中。据推测，在用醋酸铀进行整体染色过程中，糖原从细胞质中丢失，糖原区域的基质呈现出无定形特征。
