Halawani Eman M S, Alzahrani Seham S S, Gad El-Rab Sanaa M F
Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
Biomimetics (Basel). 2023 Sep 25;8(6):452. doi: 10.3390/biomimetics8060452.
The prevalence of multidrug-resistant (MDR) bacteria has recently increased dramatically, seriously endangering human health. Herein, amoxicillin (Amoxi)-conjugated gold nanoparticles (AuNPs) were created as a novel drug delivery system to overcome MDR bacteria. MDR bacteria were isolated from a variety of infection sources. Phenotype, biotype, and 16S rRNA gene analyses were used for isolate identification. Additionally, was used for the production of AuNPs. The conjugation of AuNPs with Amoxi using sodium tri-polyphosphate (TPP) as a linker to produce Amoxi-TPP-AuNPs was studied. The AuNP and Amoxi-TPP-AuNP diameters ranged from 15.99 to 24.71 nm, with spherical and hexagonal shapes. A total of 83% of amoxicillin was released from Amoxi-TPP-AuNPs after 12 h, and after 3 days, 90% of the medication was released. The Amoxi-TPP-AuNPs exhibited superior antibacterial effectiveness against MRSA and MDR strains. Amoxi-TPP-AuNPs had MICs of 3.6-8 µg mL against the tested bacteria. This is 37.5-83 fold higher compared to values reported in the literature. Amoxi-TPP-AuNPs exhibit a remarkable ability against MRSA and strains. These results demonstrate the applicability of Amoxi-TPP-AuNPs as a drug delivery system to improve therapeutic action.
多重耐药（MDR）细菌的流行率最近急剧上升，严重危及人类健康。在此，制备了阿莫西林（Amoxi）偶联的金纳米颗粒（AuNPs）作为一种新型药物递送系统来对抗MDR细菌。MDR细菌从多种感染源中分离出来。通过表型、生物型和16S rRNA基因分析进行菌株鉴定。此外， 用于制备AuNPs。研究了以三聚磷酸钠（TPP）为连接剂将AuNPs与Amoxi偶联以制备Amoxi-TPP-AuNPs。AuNP和Amoxi-TPP-AuNP的直径范围为15.99至24.71nm，形状为球形和六边形。12小时后，共有83%的阿莫西林从Amoxi-TPP-AuNPs中释放出来，3天后，90%的药物被释放。Amoxi-TPP-AuNPs对耐甲氧西林金黄色葡萄球菌（MRSA）和MDR 菌株表现出优异的抗菌效果。Amoxi-TPP-AuNPs对受试细菌的最低抑菌浓度（MIC）为3.6 - 8μg/mL。这比文献报道的值高37.5 - 83倍。Amoxi-TPP-AuNPs对MRSA和 菌株表现出显著的抗菌能力。这些结果证明了Amoxi-TPP-AuNPs作为一种药物递送系统以改善治疗作用的适用性。
