Central Research Laboratory, Institute of Medical Sciences and SUM Hospital, Siksha O Anusandhan Deemed To Be University), Kalinga Nagar, Bhubaneswar, Odisha, 751003, India.
Bioprocess Biosyst Eng. 2021 Aug;44(8):1617-1626. doi: 10.1007/s00449-021-02544-4. Epub 2021 Mar 11.
Multidrug-resistant (MDR) pathogenic bacteria have become dangerous in bringing sporadic outbreaks in public health and nosocomial spreads from the addition of antibacterials/antibiotics continually. Obviously, the pharmacy world is in search of antibacterials that would be invincible by the evolved bacteria. Green synthesis of gold-nanoparticles (AuNps) was focused on the use of aqueous chloroauric acid (HAuCl) and cell-free aqueous extract of the N-fixing cyanobacterium (blue-green alga) Anabaena spiroides collected from a brackish-water, Bay of Bengal at Puri, Odisha; green-synthesized AuNps could be used as antibacterials against MDR bacteria. The synthesized AuNps were subjected to the following characterizations, UV-Vis spectrophotometry, SEM-EDX, XRD and ART-FTIR analysis. An absorption peak at 538 nm by UV-Vis spectrophotometry and the FTIR analysis confirmed the presence of AuNps. A. spiroides-AuNps were monitored for antibacterial activities against MDR pathogenic bacterial strains isolated from clinical samples, namely, Klebsiella oxytoca, MRSA and Streptococcus pyogenes, in vitro; the individual antibiograms of those bacteria were known. The recorded MIC dose values were 25, 20 and 30 mg A. spiroides-AuNps (As-AuNps) against K. oxytoca, MRSA and S. pyogenes, in vitro, respectively. Thus, As-AuNps bear promises as possible antibacterials, in future.
多药耐药（MDR）病原菌由于抗菌药物/抗生素的不断添加，在公共卫生领域引发散发疫情和医院内传播，变得极具危害性。显然，制药界正在寻找对进化后的细菌具有不可战胜性的抗菌药物。金纳米粒子（AuNps）的绿色合成侧重于使用含水氯金酸（HAuCl）和从孟加拉湾普里的咸水收集的固氮蓝藻（蓝细菌）鱼腥藻的无细胞水提物；绿色合成的 AuNps 可作为针对 MDR 细菌的抗菌药物。合成的 AuNps 经过以下特性测试，包括紫外可见分光光度法、SEM-EDX、XRD 和 ART-FTIR 分析。紫外可见分光光度法的 538nm 吸收峰和 FTIR 分析证实了 AuNps 的存在。鱼腥藻-AuNps 被监测对从临床样本中分离出的 MDR 病原菌菌株的体外抗菌活性，这些病原菌菌株包括产酸克雷伯氏菌、耐甲氧西林金黄色葡萄球菌和化脓性链球菌，这些细菌的单独药敏谱是已知的。记录的 MIC 剂量值分别为 25、20 和 30mg 鱼腥藻-AuNps（As-AuNps）对产酸克雷伯氏菌、耐甲氧西林金黄色葡萄球菌和化脓性链球菌的体外抗菌活性。因此，As-AuNps 有望成为未来的潜在抗菌药物。
