Fernández-Lodeiro Carlos, Tambosi Reem, Fernández-Lodeiro Javier, Fernández-Lodeiro Adrián, Nuti Silvia, Ouchane Soufian, Kébaïli Nouari, Pérez-Juste Jorge, Pastoriza-Santos Isabel, Lodeiro Carlos
Departamento de Química Física, Universidade de Vigo, Campus Universitario Lagoas Marcosende, 36310 Vigo, Spain.
Galicia Sur Health Research Institute (IIS Galicia Sur), SERGAS-UVIGO, 36310 Vigo, Spain.
Nanomaterials (Basel). 2023 Oct 18;13(20):2788. doi: 10.3390/nano13202788.
In this study, we propose a novel approach for the silica coating of silver nanoparticles based on surface modification with adenosine monophosphate (AMP). Upon AMP stabilization, the nanoparticles can be transferred into 2-propanol, promoting the growth of silica on the particle surfaces through the standard Stöber process. The obtained silica shells are uniform and homogeneous, and the method allows a high degree of control over shell thickness while minimizing the presence of uncoated NPs or the negligible presence of core-free silica NPs. In addition, AMP-functionalized AgNPs could be also coated with a mesoporous silica shell using cetyltrimethylammonium chloride (CTAC) as a template. Interestingly, the thickness of the mesoporous silica coating could be tightly adjusted by either the silica precursor concentration or by varying the CTAC concentration while keeping the silica precursor concentration constant. Finally, the influence of the silica coating on the antimicrobial effect of AgNPs was studied on Gram-negative bacteria ( and ) and under different bacterial growth conditions, shedding light on their potential applications in different biological environments.
在本研究中，我们提出了一种基于用单磷酸腺苷（AMP）进行表面改性的银纳米颗粒二氧化硅包覆新方法。在AMP稳定化后，纳米颗粒可转移到2-丙醇中，通过标准的Stöber工艺促进颗粒表面二氧化硅的生长。所获得的二氧化硅壳均匀且同质，该方法能够高度控制壳厚度，同时使未包覆的纳米颗粒的存在降至最低或使无核二氧化硅纳米颗粒的存在可忽略不计。此外，使用十六烷基三甲基氯化铵（CTAC）作为模板，AMP功能化的银纳米颗粒也可以包覆中孔二氧化硅壳。有趣的是，在保持二氧化硅前驱体浓度恒定的同时，通过改变二氧化硅前驱体浓度或改变CTAC浓度，可以严格调节中孔二氧化硅涂层的厚度。最后，研究了二氧化硅涂层对银纳米颗粒对革兰氏阴性菌（和）抗菌效果的影响以及在不同细菌生长条件下的影响，揭示了它们在不同生物环境中的潜在应用。
