University of Modena and Reggio Emilia, Reggio Emilia, Italy; NoviNano Lab LLC, Lviv, Ukraine; Department of Photonics, Lviv Polytechnic National University, Lviv, Ukraine.
Sumy State University, Sumy, Ukraine; Osteoplant R&D, Debice, Poland.
Nanomedicine. 2019 Oct;21:102036. doi: 10.1016/j.nano.2019.102036. Epub 2019 Jun 19.
In this paper, the effect of femtosecond laser nanotexturing of surfaces of Ti6Al4V and Zr implants on their biological compatibility is presented and discussed. Highly regular and homogeneous nanostructures with sub-micrometer period were imprinted on implant surfaces. Surfaces were morphologically and chemically investigated by SEM and XPS. HDFa cell lines were used for toxicity and cell viability tests, and subcutaneous implantation was applied to characterize tissue response. HDFa proliferation and in vivo experiments evidenced the strong influence of the surface topography compared to the effect of the surface elemental composition (metal or alloy). The effect of protein adsorption from blood plasma on cell proliferation is also discussed.
本文介绍并讨论了飞秒激光对 Ti6Al4V 和 Zr 植入物表面纳米纹理处理对其生物相容性的影响。在植入物表面上形成了具有亚微米周期的高度规则和均匀的纳米结构。通过 SEM 和 XPS 对表面形貌和化学性质进行了研究。使用 HDFa 细胞系进行毒性和细胞活力测试，并进行皮下植入以表征组织反应。HDFa 增殖和体内实验证明了表面形貌与表面元素组成（金属或合金）相比对细胞增殖的强烈影响。还讨论了从血浆中吸附蛋白质对细胞增殖的影响。
