Lu Qi, Jin Longfei, Wang Peng, Liu Feng, Huang Bei, Wen Mingxia, Wu Shaohui
Zhejiang Citrus Research Institute, Taizhou 318026, China.
College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China.
J Fungi (Basel). 2023 Sep 29;9(10):983. doi: 10.3390/jof9100983.
Protein hydrolysates (PHs) and arbuscular mycorrhizal fungi (AMF) are environmentally friendly biostimulants that effectively promote crop growth and alleviate the damage from abiotic stress. However, the physiological and molecular regulatory mechanisms are still unclear. This study aimed to explore the effects of PHs and AMF on growth, mineral nutrient absorption, and expression of and in Goutoucheng () under salt stress. Results showed that PH application and AMF inoculation significantly promoted plant growth and enhanced mineral element absorption and sodium effluxion in citrus under salt stress. The biomass, root activity, leaves mineral nutrition contents in PHs, AMF, and combined (PHs and AMF) treatments were significantly higher than those of control. Leaves sodium content in three treatments was significantly lower than in the control. AMF and combined treatments showed dominant effects than PHs alone. Besides, PHs interacted with AMF on growth, nutrient absorption, and sodium effluxion. Importantly, AMF and PHs induced stress-responsive genes. , , , and expression in PHs and AMF treatments was significantly higher than control. Thus, it was concluded that AMF and PHs enhanced the salt tolerance of citrus by promoting nutrient absorption and sodium effluxion via up-regulating the expression of and . The mixed application of PHs and AMF had a better effect.
蛋白水解物（PHs）和丛枝菌根真菌（AMF）是环境友好型生物刺激剂，可有效促进作物生长并减轻非生物胁迫造成的损害。然而，其生理和分子调控机制仍不清楚。本研究旨在探讨PHs和AMF对盐胁迫下狗头橙（）生长、矿质养分吸收以及 和 表达的影响。结果表明，在盐胁迫下，施用PHs和接种AMF显著促进了柑橘的生长，增强了矿质元素吸收和钠外流。PHs、AMF及组合（PHs和AMF）处理的生物量、根系活力、叶片矿质营养含量均显著高于对照。三种处理的叶片钠含量均显著低于对照。AMF及组合处理比单独施用PHs表现出更显著的效果。此外，PHs与AMF在生长、养分吸收和钠外流方面存在相互作用。重要的是，AMF和PHs诱导了胁迫响应基因。PHs和AMF处理中 、 、 和 的表达显著高于对照。因此，得出结论：AMF和PHs通过上调 和 的表达促进养分吸收和钠外流，从而增强了柑橘的耐盐性。PHs和AMF混合施用效果更佳。
