Regenmed Ltd., Bratislava, Slovak Republic.
Physiol Res. 2023 Oct 27;72(S3):S299-S307. doi: 10.33549/physiolres.935211.
Congenital anomalies, diseases, and injuries may result in osteochondral damage. Recently, a big hope has been given to somatic stem cells (SSCs) which are characterized as undifferentiated cells with an ability of long-term self-renewing and plasticity. They are adherent with a fibroblast-like morphology in vitro and express various surface markers (e.g. CD29, CD73, CD90, and CD105), but they are negative for CD31, CD34, CD45, and HLA-DR. SSCs secrete various bioactive molecules, which are involved in processes of regeneration. The main goal of the present study was the characterization and comparison of biological properties of SSCs obtained from adipose tissue, dental pulp, and urine concerning osteochondral regeneration. SSCs were maintained in an appropriate growth medium up to the third passage and were analyzed by light and electron microscope. The immunophenotype was analyzed by flow cytometry. The kinetics of proliferation was measured by MTT assay. Human Cytokine/Chemokine Multiplex Assay was used, and SSCs secretory profile was measured by Luminex MAGPIX® Instrument. Pellet cultures and a chondrogenic medium were used to induce chondrogenic differentiation. Osteogenic differentiation was induced by the osteogenic medium. Chondrogenic and osteogenic differentiation was analyzed by real-time PCR. SSCs had similar fibroblast-like morphology. They have similar kinetics of proliferation. SSCs shared the expression CD29, CD44, CD73, CD90, and CD105. They lack expression of CD29 and CD34. SSCs secerned similar levels of IL10 and IL18 while differing in IFN-gamma, IL6, IL8, MCP-1, and RANTES production. SSCs possess a similar capacity for chondrogenic differentiation but slightly differ in osteogenic differentiation. In conclusion, it can be emphasized that SSCs from adipose tissue, dental pulp, and urine share the majority of cellular characteristics typical for SSCs and have great potential to be used in osteochondral tissue regeneration.
先天性异常、疾病和损伤可能导致骨软骨损伤。最近，人们对体干细胞（SSCs）寄予了厚望，这些细胞具有未分化的特征，具有长期自我更新和可塑性。在体外，它们呈纤维母细胞样形态，表达各种表面标志物（如 CD29、CD73、CD90 和 CD105），但它们对 CD31、CD34、CD45 和 HLA-DR 呈阴性。SSCs 分泌各种生物活性分子，参与再生过程。本研究的主要目的是表征和比较从脂肪组织、牙髓和尿液中获得的 SSCs 在骨软骨再生方面的生物学特性。SSCs 在适当的生长培养基中维持至第 3 代，并通过光镜和电子显微镜进行分析。通过流式细胞术分析免疫表型。通过 MTT 测定法测量增殖动力学。使用人细胞因子/趋化因子多重分析试剂盒，通过 Luminex MAGPIX®仪器测量 SSCs 的分泌谱。使用 pellet 培养和软骨形成培养基诱导软骨分化。通过成骨培养基诱导成骨分化。通过实时 PCR 分析软骨和成骨分化。SSCs 具有相似的成纤维细胞样形态。它们具有相似的增殖动力学。SSCs 共同表达 CD29、CD44、CD73、CD90 和 CD105。它们缺乏 CD29 和 CD34 的表达。SSCs 分泌的 IL10 和 IL18 水平相似，但 IFN-γ、IL6、IL8、MCP-1 和 RANTES 的产生水平不同。SSCs 具有相似的软骨分化能力，但在成骨分化方面略有不同。总之，可以强调的是，来自脂肪组织、牙髓和尿液的 SSCs 具有大多数细胞特征，这些特征与 SSCs 典型特征相似，具有很大的潜力用于骨软骨组织再生。
