Xing Fei, Yin Hua-Mo, Zhe Man, Xie Ji-Chang, Duan Xin, Xu Jia-Zhuang, Xiang Zhou, Li Zhong-Ming
Orthopedic Research Institute, Department of Orthopedics, West China Hospital, Sichuan University, No. 37 Guoxue Lane, Chengdu 610041, China.
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.
Pharmaceutics. 2022 Jul 8;14(7):1437. doi: 10.3390/pharmaceutics14071437.
3D-printing technology can be used to construct personalized bone substitutes with customized shapes, but it cannot regulate the topological morphology of the scaffold surface, which plays a vital role in regulating the biological behaviors of stem cells. In addition, stem cells are able to sense the topographical and mechanical cues of surface of scaffolds by mechanosensing and mechanotransduction. In our study, we fabricated a 3D-printed poly(ε-caprolactone) (PCL) scaffold with a nanotopographical surface and loaded it with urine-derived stem cells (USCs) for application of bone regeneration. The topological 3D-printed PCL scaffolds (TPS) fabricated by surface epiphytic crystallization, possessed uniformly patterned nanoridges, of which the element composition and functional groups of nanoridges were the same as PCL. Compared with bare 3D-printed PCL scaffolds (BPS), TPS have a higher ability for protein adsorption and mineralization in vitro. The proliferation, cell length, and osteogenic gene expression of USCs on the surface of TPS were significantly higher than that of BPS. In addition, the TPS loaded with USCs exhibited a good ability for bone regeneration in cranial bone defects. Our study demonstrated that nanotopographical 3D-printed scaffolds loaded with USCs are a safe and effective therapeutic strategy for bone regeneration.
3D打印技术可用于构建具有定制形状的个性化骨替代物，但它无法调节支架表面的拓扑形态，而支架表面拓扑形态在调节干细胞的生物学行为中起着至关重要的作用。此外，干细胞能够通过机械传感和机械转导感知支架表面的拓扑和机械信号。在我们的研究中，我们制备了一种具有纳米拓扑表面的3D打印聚己内酯（PCL）支架，并将尿液来源的干细胞（USCs）接种于其上用于骨再生。通过表面附生结晶制备的拓扑3D打印PCL支架（TPS）具有均匀图案化的纳米脊，其纳米脊的元素组成和官能团与PCL相同。与裸3D打印PCL支架（BPS）相比，TPS在体外具有更高的蛋白质吸附和矿化能力。USCs在TPS表面的增殖、细胞长度和成骨基因表达均显著高于BPS。此外，接种USCs的TPS在颅骨缺损中表现出良好的骨再生能力。我们的研究表明，负载USCs的纳米拓扑3D打印支架是一种安全有效的骨再生治疗策略。
