Luo Xiaoping, Wang Shuyi, Feng Ying, Wang Penglong, Gong Gaowa, Guo Tianlong, Feng Xingang, Yang Xiaoye, Li Junyan
Key Laboratory of Grass-Feeding Livestock Healthy Breeding and Livestock Product Quality Control, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot 010030, China.
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China.
Animals (Basel). 2023 Jun 1;13(11):1841. doi: 10.3390/ani13111841.
poses a severe hazard to the healthy development of the sheep industry and threatens the welfare of sheep. Ivermectin is the primary anthelmintic used for the prevention and treatment of parasitism. However, the widespread and uncontrolled application of ivermectin has resulted in the development and spread of resistant strains of . P-glycoprotein (P-gp) plays important roles in the pharmacology and toxicology of ivermectin, and changes in expression levels can be used to analyze the resistance of to ivermectin. This study aimed to analyze the effects of ivermectin on expression in L3 larvae isolated from China and to evaluate whether changes in expression levels can be used to analyze resistant strains. In the absence of drug treatment, the ivermectin-resistant strains isolated in China showed increased expression of 11 ( < 0.01) compared with sensitive strains from elsewhere, whereas the expressions of 2 and 9.1 were downregulated ( < 0.01). When the same strain was compared before and after drug treatment, obvious differences in expression were observed between the different strains. Ivermectin-induced expression was found to be very complex among the L3 larvae of different strains. In addition, it was confirmed that using to determine ivermectin resistance in strains from different geographic environments can yield different results.
对养羊业的健康发展构成严重危害，并威胁到羊的福利。伊维菌素是用于预防和治疗寄生虫病的主要驱虫药。然而，伊维菌素的广泛和无节制应用导致了抗药菌株的产生和传播。P-糖蛋白（P-gp）在伊维菌素的药理学和毒理学中起重要作用，其表达水平的变化可用于分析对伊维菌素的抗性。本研究旨在分析伊维菌素对从中国分离的L3幼虫中 表达的影响，并评估 表达水平的变化是否可用于分析抗药菌株.在中国分离的伊维菌素抗药菌株在未进行药物处理时，与其他地方的敏感菌株相比，11的表达增加（<0.01），而2和9.1的表达下调（<0.01）。当对同一菌株进行药物处理前后比较时，不同菌株之间观察到明显的表达差异。发现伊维菌素诱导的不同菌株L3幼虫中的 表达非常复杂。此外，可以确定的是以 来确定来自不同地理环境的 菌株对伊维菌素抗性可能会产生不同结果。
