College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.
College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.
Anal Biochem. 2023 Dec 15;683:115370. doi: 10.1016/j.ab.2023.115370. Epub 2023 Oct 27.
As the most well-known analytical tool, the thermometer has been extended to the field of biological analysis based on the photothermal effect. Herein, isoniazide modified Ag nanoparticles were prepared as nanolabels to build an immunoassay. The nanoparticles were characterized by transmission electron microscope (TEM), dynamic laser scattering (DLS), X-ray powder diffraction (XRD), and Fourier transform infrared (FT-IR). When the target protein was present, the sandwich immunoassay was developed and the photothermal reaction was triggered by isoniazide modified Ag nanoparticles. As a reducing agent, isoniazide is used to transform phosphomolybdic acid hydrate into molybdenum blue solution. And molybdenum blue had good photothermal stability and high photothermal conversion efficiency. The temperature variation of molybdenum blue solution showed a positive correlation with the concentration of carcinoembryonic antigen (CEA). Thus, the target protein of CEA was quantitative detection by thermometer. The linear response range is 0.1 ng mL to 40 ng mL, and the detection limit is 0.08 ng mL. Moreover, the proposed protocol had satisfactory selectivity, accuracy, and reproducibility.
作为最著名的分析工具，基于光热效应，温度计已经扩展到生物分析领域。在这里，异烟肼修饰的 Ag 纳米粒子被制备为纳米标签以构建免疫分析。通过透射电子显微镜 (TEM)、动态激光散射 (DLS)、X 射线粉末衍射 (XRD) 和傅里叶变换红外 (FT-IR) 对纳米粒子进行了表征。当存在目标蛋白时，开发了夹心免疫测定，并通过异烟肼修饰的 Ag 纳米粒子触发光热反应。作为还原剂，异烟肼将磷钼酸水合物转化为钼蓝溶液。并且钼蓝具有良好的光热稳定性和高光热转换效率。钼蓝溶液的温度变化与癌胚抗原 (CEA) 的浓度呈正相关。因此，通过温度计对 CEA 的靶蛋白进行定量检测。线性响应范围为 0.1ng mL 至 40ng mL，检测限为 0.08ng mL。此外，该方案具有令人满意的选择性、准确性和重现性。
