School of Public Health, Bengbu Medical College, Bengbu, PR China.
Department of Biochemistry and Molecular Biology, School of Laboratory Medicine; Anhui Province Key Laboratory of Immunology in Chronic Diseases, Bengbu Medical College, Bengbu, PR China.
J Nutr Biochem. 2024 Jan;123:109501. doi: 10.1016/j.jnutbio.2023.109501. Epub 2023 Oct 25.
Perfluorooctanoic acid (PFOA) is a persistent environmental pollutant that can accumulate in the kidneys and eventually cause kidney damage. Rutin (RUTIN) is a natural flavonoid with multiple biological activities, and its use in against kidney damage has been widely studied in recent years. It is not yet known whether rutin protects against kidney damage caused by PFOA. In this study, 30 ICR mice were randomly divided into three groups: CTRL group, PFOA group and PFOA+RUTIN group. The mice were fed continuously by gavage for 28 days. Renal pathological changes were assessed by HE and PASM staining, and serum renal function and lipid indicators were measured. RNA-seq and enrichment analysis using GO, KEGG and PPI to detect differential expression of genes in treatment groups. Kidney tissue protein expression was determined by Western blot. Research has shown that rutin can improve glomerular and tubular structural damage, and increase serum CREA, HDL-C levels and decrease LDH, LDL-C levels. The expression of AQP1 and ACOT1 was up-regulated after rutin treatment. Transcriptomic analysis indicated that PFOA and rutin affect the transcriptional expression of genes related to lipid metabolism and oxidative stress, and may affected by PI3K-Akt, PPAR, NRF2/KEAP1 signaling pathways. In conclusion, rutin ameliorated renal damage caused by PFOA exposure, and this protective effect may be exerted by ameliorating oxidative stress and regulating lipid metabolism.
全氟辛酸（PFOA）是一种持久性的环境污染物，可在肾脏中积累，最终导致肾脏损伤。芦丁（RUTIN）是一种具有多种生物活性的天然类黄酮，近年来其在防治肾脏损伤方面的应用已得到广泛研究。但目前尚不清楚芦丁是否能预防 PFOA 引起的肾脏损伤。在这项研究中，将 30 只 ICR 小鼠随机分为三组：对照组（CTRL 组）、PFOA 组和 PFOA+RUTIN 组。通过灌胃连续喂养 28 天。通过 HE 和 PASM 染色评估肾脏病理变化，测量血清肾功能和脂质指标。使用 GO、KEGG 和 PPI 进行 RNA-seq 和富集分析，以检测治疗组中基因的差异表达。通过 Western blot 测定肾脏组织蛋白表达。研究表明，芦丁可改善肾小球和肾小管结构损伤，增加血清 CREA、HDL-C 水平，降低 LDH、LDL-C 水平。芦丁处理后 AQP1 和 ACOT1 的表达上调。转录组分析表明，PFOA 和芦丁影响与脂质代谢和氧化应激相关的基因的转录表达，可能受 PI3K-Akt、PPAR、NRF2/KEAP1 信号通路影响。综上所述，芦丁可改善 PFOA 暴露引起的肾脏损伤，这种保护作用可能是通过改善氧化应激和调节脂质代谢来实现的。
