Gidda J S, Buyniski J P
Am J Physiol. 1986 Dec;251(6 Pt 1):G779-85. doi: 10.1152/ajpgi.1986.251.6.G779.
This study examined the role of cholinergic mechanisms in esophageal smooth muscle of anesthetized opossums. Swallow-induced motility was recorded manometrically before and after treatment with physostigmine or metoclopramide. At the 5-cm site above lower esophageal sphincter (LES) the latency, duration, and amplitude of swallow-evoked contractions were 2.1 +/- 0.12 (SE) s, 1.8 +/- 0.31 s, and 74.5 +/- 6.1 mmHg, respectively. At the 1-cm site the respective values were 4.6 +/- 0.38 s, 3.8 +/- 0.24 s, and 42.0 +/- 4.6 mmHg. The calculated speed of peristalsis was 1.72 +/- 0.21 cm/s. After intravenous treatment with the physostigmine (100 micrograms X kg-1 X h-1), each swallow produced contractions that were simultaneous, repetitive, and of long duration. The values of duration and amplitude of contraction at the 5- and 1-cm site after physostigmine treatment were significantly higher than controls (P less than 0.01). There was a significant decrease in the latency of contraction at all sites. This resulted in a significant faster speed of peristalsis. Atropine (30 micrograms/kg iv) reversed the influence of physostigmine, resulting in a significantly slower speed of peristalsis. Metoclopramide (10 mg/kg iv) resulted in several changes in swallow-evoked contractions similar to those observed after physostigmine treatment. These data suggest that alteration in cholinergic mechanisms results in disordered esophageal peristalsis in the opossum.
本研究探讨了胆碱能机制在麻醉负鼠食管平滑肌中的作用。在用毒扁豆碱或甲氧氯普胺治疗前后，通过压力测定法记录吞咽诱发的运动。在下食管括约肌（LES）上方5厘米处，吞咽诱发收缩的潜伏期、持续时间和幅度分别为2.1±0.12（SE）秒、1.8±0.31秒和74.5±6.1毫米汞柱。在1厘米处，相应的值分别为4.6±0.38秒、3.8±0.24秒和42.0±4.6毫米汞柱。计算得出的蠕动速度为1.72±0.21厘米/秒。静脉注射毒扁豆碱（100微克·千克⁻¹·小时⁻¹）后，每次吞咽都会产生同步、重复且持续时间长的收缩。毒扁豆碱治疗后，5厘米和1厘米处收缩的持续时间和幅度值显著高于对照组（P＜0.01）。所有部位收缩的潜伏期均显著缩短。这导致蠕动速度显著加快。阿托品（30微克/千克静脉注射）可逆转毒扁豆碱的影响，使蠕动速度显著减慢。甲氧氯普胺（10毫克/千克静脉注射）导致吞咽诱发收缩出现了一些与毒扁豆碱治疗后相似的变化。这些数据表明，胆碱能机制的改变导致负鼠食管蠕动紊乱。
