Eilat-Adar Sigal, Kassem Eias, Sindiani Mahmood, Ben-Zaken Sigal
Levinsky-Wingate Academic College, Wingate Campus, Netanya 4290200, Israel.
Hillel-Yaffe Medical Center, Hadera 3810000, Israel.
Children (Basel). 2023 Sep 27;10(10):1610. doi: 10.3390/children10101610.
Both the IGF1 axis and hypovitaminosis D play a role in childhood obesity, either as a cause or a causality. While some studies suggest an interrelation between vitamin D status, IGF1, and obesity, this mechanism remains obscure. The aim of this study, therefore, was to explore associations between four genetic polymorphisms in the IGF1 axis in hypovitaminosis D-related obesity. The study included 116 pre-pubertal Israeli Arab children (52 girls), mean age 9.4 ± 2.6. Serum 25(OH)D was measured and anthropometric measures were obtained. Genomic DNA was extracted from peripheral EDTA-treated anti-coagulated blood using a standard protocol. Genotypes were determined using the Taqman allelic discrimination assay. The IGF genetic score was computed according to the additive genetic score model. A moderate-to-high negative correlation (r = 0.580, p < 0.05) was seen between the vitamin D status and body mass index (BMI) percentile of participants with high GS. Yet, no correlations were seen between vitamin D status and BMI percentile for participants with a low-to-moderate genetic score (GS) (GS ≤ 2). These results suggest that IGF1 genetic scores associated with elevated circulating IGF1 may indicate a tendency toward developing hypovitaminosis D-associated obesity.
IGF1轴和维生素D缺乏在儿童肥胖中均起作用，无论是作为原因还是因果关系。虽然一些研究表明维生素D状态、IGF1和肥胖之间存在相互关系，但这种机制仍不清楚。因此，本研究的目的是探讨维生素D缺乏相关肥胖中IGF1轴的四种基因多态性之间的关联。该研究纳入了116名青春期前的以色列阿拉伯儿童（52名女孩），平均年龄9.4±2.6岁。测量血清25(OH)D并获取人体测量数据。使用标准方案从外周EDTA处理的抗凝血中提取基因组DNA。使用Taqman等位基因鉴别分析确定基因型。根据加性遗传评分模型计算IGF基因评分。在高基因评分参与者的维生素D状态与体重指数（BMI）百分位数之间观察到中度至高度负相关（r = 0.580，p < 0.05）。然而，在低至中度基因评分（GS）（GS≤2）的参与者中，未观察到维生素D状态与BMI百分位数之间的相关性。这些结果表明，与循环IGF1升高相关的IGF1基因评分可能表明有发展为维生素D缺乏相关肥胖的倾向。
