Negut Irina, Gradisteanu-Pircalabioru Gratiela, Dinu Mihaela, Bita Bogdan, Parau Anca Constantina, Grumezescu Valentina, Ristoscu Carmen, Chifiriuc Mariana Carmen
National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Street, P.O. Box MG 36, 077125 Magurele, Romania.
eBio-Hub Research Center, University Politehnica of Bucharest-CAMPUS, 6 Iuliu Maniu Boulevard, 061344 Bucharest, Romania.
Biomedicines. 2023 Oct 12;11(10):2772. doi: 10.3390/biomedicines11102772.
The use of MAPLE synthesized thin films based on BG and VD3 for improving the osseointegration and corrosion protection of Ti-like implant surfaces is reported. The distribution of chemical elements and functional groups was shown by FTIR spectrometry; the stoichiometry and chemical functional integrity of thin films after MAPLE deposition was preserved, optimal results being revealed especially for the BG+VD3_025 samples. The morphology and topography were examined by SEM and AFM, and revealed surfaces with many irregularities, favoring a good adhesion of cells. The thin films' cytotoxicity and biocompatibility were evaluated in vitro at the morphological, biochemical, and molecular level. Following incubation with HDF cells, BG57+VD3_ 025 thin films showed the best degree of biocompatibility, as illustrated by the viability assay values. According to the LDH investigation, all tested samples had higher values compared to the unstimulated cells. The evaluation of cell morphology was performed by fluorescence microscopy following cultivation of HDF cells on the obtained thin films. The cultivation of HDF's on the thin films did not induce major cellular changes. Cells cultured on the BG57+VD3_025 sample had similar morphology to that of unstimulated control cells. The inflammatory profile of human cells cultured on thin films obtained by MAPLE was analyzed by the ELISA technique. It was observed that the thin films did not change the pro- and anti-inflammatory profile of the HDF cells, the IL-6 and IL-10 levels being similar to those of the control sample. The wettability of the MAPLE thin films was investigated by the sessile drop method. A contact angle of 54.65° was measured for the sample coated with BG57+VD3_025. Electrochemical impedance spectroscopy gave a valuable insight into the electrochemical reactions occurring on the surface.
报道了基于生物玻璃（BG）和维生素D3（VD3）通过基质辅助脉冲激光蒸发（MAPLE）合成薄膜以改善类钛植入物表面骨整合和腐蚀防护的应用。傅里叶变换红外光谱法（FTIR）显示了化学元素和官能团的分布；MAPLE沉积后薄膜的化学计量和化学功能完整性得以保留，特别是对于BG + VD3_025样品显示出最佳结果。通过扫描电子显微镜（SEM）和原子力显微镜（AFM）检查了形态和表面形貌，发现表面存在许多不规则之处，有利于细胞的良好粘附。在形态、生化和分子水平上对薄膜的细胞毒性和生物相容性进行了体外评估。与人类皮肤成纤维细胞（HDF）孵育后，BG57 + VD3_025薄膜显示出最佳的生物相容性，这由活力测定值表明。根据乳酸脱氢酶（LDH）研究，与未刺激的细胞相比，所有测试样品的值都更高。在所得薄膜上培养HDF细胞后，通过荧光显微镜对细胞形态进行了评估。HDF细胞在薄膜上的培养未引起主要的细胞变化。在BG57 + VD3_025样品上培养的细胞与未刺激的对照细胞具有相似的形态。通过酶联免疫吸附测定（ELISA）技术分析了在通过MAPLE获得的薄膜上培养的人类细胞的炎症特征。观察到薄膜未改变HDF细胞的促炎和抗炎特征，白细胞介素-6（IL-6）和白细胞介素-10（IL-10）水平与对照样品相似。通过静滴法研究了MAPLE薄膜的润湿性。测量了涂覆有BG57 + VD3_025的样品的接触角为54.65°。电化学阻抗谱对表面发生的电化学反应提供了有价值的见解。
