Lapsina Sandra, Nagler Nicole, Müller Simon Franz, Holtdirk Annette, Kottmann Tanja, Müller Elisabeth, Schäfer Ingo
LABOKLIN GmbH and Co. KG, Steubenstraße 4, 97688 Bad Kissingen, Germany.
Dr. MED. Kottmann-Clinical Research Organization, Beverstraße 64, 59077 Hamm, Germany.
Animals (Basel). 2023 Oct 13;13(20):3202. doi: 10.3390/ani13203202.
Fibroblast growth factor-23 (FGF-23) is a phosphaturic hormone used to monitor chronic kidney disease (CKD) in humans. The aims of this study were (1) to determine the intra- and interassay precision of the FGF-23 concentrations in dogs as measured via the Kainos ELISA FGF-23 kit, (2) to calculate a reference interval, and (3) to assess the correlation of the FGF-23 concentration with the hematological and biochemical parameters. The coefficient of variation was below 15% for both the intra- and interassay precision, indicating good reproducibility. The reference interval ranged between 95.8 (90% confidence interval: 44.6; 139.2) and 695.1 pg/mL (598.7; 799.1) based on 136 clinically healthy dogs, classified as such according to the information of treating veterinarians as well as the unremarkable results of hematology and biochemistry. The FGF-23 concentration differed significantly between dogs aged <9 and ≥9 years ( = 0.045). Four groups of 10 dogs each were retrospectively formed based on the creatinine concentration classification according to the IRIS staging. Correlation was the strongest for the renal parameters. Statistically significant differences in the FGF-23 concentration were demonstrated between the study groups I and III ( 0.001), I and IV ( 0.001), and II and IV ( = 0.005). There was a trend for a rising FGF-23 concentration in older dogs. Due to the wide reference interval, diagnostic cut-offs and/or subject-based FGF-23 reference values in each dog are needed for monitoring and clinical interpretation.
成纤维细胞生长因子23（FGF - 23）是一种用于监测人类慢性肾脏病（CKD）的排磷激素。本研究的目的是：（1）确定通过凯诺斯ELISA FGF - 23试剂盒测定的犬FGF - 23浓度的批内和批间精密度；（2）计算参考区间；（3）评估FGF - 23浓度与血液学和生化参数的相关性。批内和批间精密度的变异系数均低于15%，表明具有良好的重复性。基于136只临床健康犬，参考区间为95.8（90%置信区间：44.6；139.2）至695.1 pg/mL（598.7；799.1），这些犬是根据主治兽医的信息以及血液学和生物化学检查结果无异常而分类为临床健康的。9岁以下和≥9岁犬的FGF - 23浓度存在显著差异（P = 0.045）。根据IRIS分期，根据肌酐浓度分类回顾性地将犬分为四组，每组10只。与肾脏参数的相关性最强。研究组I和III（P < 0.001）、I和IV（P < 0.001）以及II和IV（P = 0.005）之间的FGF - 23浓度存在统计学显著差异。老年犬的FGF - 23浓度有上升趋势。由于参考区间较宽，监测和临床解读需要每个犬的诊断临界值和/或基于个体的FGF - 23参考值。
