Hiżewska Ligia, Osiak-Wicha Cezary, Tomaszewska Ewa, Muszyński Siemowit, Dobrowolski Piotr, Andres Krzysztof, Schwarz Tomasz, Arciszewski Marcin B
Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland.
Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland.
Animals (Basel). 2023 Oct 21;13(20):3292. doi: 10.3390/ani13203292.
In this study, a morphometric analysis of morphological changes in the layers of the small intestine (duodenum and jejunum) and liver occurring during the hatching period (week 0) and postnatal development (weeks 1, 3, 6, and 8) was performed in geese. For this purpose, the staining of samples obtained from tissues collected from geese after culling was carried out. Staining was performed using the Goldner method to visualize all layers of the intestine for morphometric measurements. Our analysis focused mainly on traits such as the thickness of the mucosal, submucosal, and muscular layers, as well as traits related to intestinal absorption, such as the height and width of intestinal villi and crypts. Additionally, we also took into account the number of mononuclear and binucleate hepatocytes and other cells present in the liver. After analyzing the results, an increase in most traits was found during the development of the animals, with slight differences between the sections of the duodenum and jejunum. An interesting phenomenon was also noticed-the greatest increase in most traits was observed between the 3rd and 6th week of life, which coincides with the time of feed change. We hope that our work will highlight how important the digestive system is for birds because research on this topic is limited.
在本研究中，对鹅在孵化期（第0周）和出生后发育阶段（第1、3、6和8周）小肠（十二指肠和空肠）及肝脏各层发生的形态变化进行了形态计量分析。为此，对扑杀后的鹅所采集组织的样本进行了染色。使用戈德纳方法进行染色，以便观察肠道的所有层次用于形态计量测量。我们的分析主要集中在诸如黏膜层、黏膜下层和肌层的厚度等特征上，以及与肠道吸收相关的特征，如肠绒毛和隐窝的高度与宽度。此外，我们还考虑了肝脏中单核和双核肝细胞及其他细胞的数量。分析结果后发现，在动物发育过程中大多数特征都有所增加，十二指肠和空肠各段之间存在细微差异。还注意到一个有趣的现象——大多数特征在出生后第3周和第6周之间增加最为显著，这与饲料更换的时间相吻合。我们希望我们的工作能凸显消化系统对鸟类的重要性，因为关于这一主题的研究有限。
