Zhang Guohui, Li Rongyu, Wu Xiaomao, Li Ming
Institute of Crop Protection, College of Agriculture, Guizhou University, Guiyang 550025, China.
College of Life and Health Science, Kaili University, Kaili 556000, China.
Microorganisms. 2023 Sep 26;11(10):2395. doi: 10.3390/microorganisms11102395.
This study aims to determine the effects of the natural product aloesin against . The results exposed that aloesin had a high inhibitory effect on appressorium formation (the EC value was 175.26 μg/mL). Microscopic examination revealed that 92.30 ± 4.26% of spores could be broken down by 625.00 μg/mL of aloesin, and the formation rate of appressoria was 4.74 ± 1.00% after 12 h. mycelial growth was weaker than that on the control. The enzyme activity analysis results indicated that aloesin inhibited the activities of polyketolase (PKS), laccase (LAC), and chain-shortening catalytic enzyme (Aayg1), which are key enzymes in melanin synthesis. The inhibition rate by aloesin of PKS, LAC, and Aayg1 activity was 32.51%, 33.04%, and 43.38%, respectively. The proteomic analysis showed that actin expression was downregulated at 175.62 μg/mL of aloesin, which could reduce actin bundle formation and prevent the polar growth of hyphae in . This is the first report showing that aloesin effectively inhibits conidia morphology and appressorium formation in .
本研究旨在确定天然产物芦荟素对……的影响。结果表明，芦荟素对附着胞形成具有高度抑制作用（EC值为175.26μg/mL）。显微镜检查显示，625.00μg/mL的芦荟素可分解92.30±4.26%的孢子，12小时后附着胞形成率为4.74±1.00%。……的菌丝体生长比对照弱。酶活性分析结果表明，芦荟素抑制了聚酮合酶（PKS）、漆酶（LAC）和链缩短催化酶（Aayg1）的活性，这些酶是黑色素合成中的关键酶。芦荟素对PKS、LAC和Aayg1活性的抑制率分别为32.51%、33.04%和43.38%。蛋白质组学分析表明，在175.62μg/mL的芦荟素作用下，肌动蛋白表达下调，这可能会减少肌动蛋白束的形成，并阻止……中菌丝的极性生长。这是首次报道芦荟素有效抑制……中的分生孢子形态和附着胞形成。
