Hernández Flores José Luis, Martínez Yomaiko Javier, Ramos López Miguel Ángel, Saldaña Gutierrez Carlos, Reyes Aldo Amaro, Armendariz Rosales Mariem Monserrat, Cortés Pérez Maraly Jazmin, Mendoza Mayela Fosado, Ramírez Ramírez Joanna, Zavala Grecia Ramírez, Tovar Becerra Paola Lizeth, Valdez Santoyo Laila, Villasana Rodríguez Karen, Rodríguez Morales José Alberto, Campos Guillén Juan
Centro de Investigación y de Estudios Avanzados del IPN, Irapuato 6824, Mexico.
Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas S/N, Querétaro 76010, Mexico.
Microorganisms. 2023 Oct 4;11(10):2491. doi: 10.3390/microorganisms11102491.
The Cp1 strain was isolated from seeds of R. & P. cultivated in Michoacan, Mexico. Genetic and ecological role analyses were conducted for better characterization. The results show that genome has a length of 4.7 Mbp with 56.22% G + C and an IncF plasmid of 128 Kbp with 52.51% G + C. Furthermore, pathogenicity test revealed nonpathogenic traits confirmed by the absence of specific virulence-related genes. Interestingly, when fungal inhibitory essays were carried out, the bacterial synthesis of volatile organic compounds (VOCs) with antifungal activity showed that . and were inhibited by 87.45% and 77.24%, respectively. Meanwhile, , , and demonstrated a mean radial growth inhibition of 52.79%, 40.82%, and 55.40%, respectively. The lowest inhibition was by with 10.64%. The VOCs' characterization by headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS) revealed 65 potential compounds. Some of the compounds identified with high relative abundance were ketones (22.47%), represented by 2-butanone, 3-hydroxy (13.52%), and alcohols (23.5%), represented by ethanol (5.56%) and 1-butanol-3-methyl (4.83%). Our findings revealed, for the first time, that Cp1 associated with seeds possesses potential traits indicating that it could serve as an effective biocontrol.
Cp1菌株是从墨西哥米却肯州种植的R. & P.种子中分离出来的。为了更好地表征该菌株，进行了遗传和生态作用分析。结果表明，该基因组长度为4.7 Mbp，G + C含量为56.22%，还有一个128 Kbp的IncF质粒，G + C含量为52.51%。此外，致病性测试显示该菌株无致病性，这一点通过缺乏特定的毒力相关基因得到证实。有趣的是，在进行真菌抑制试验时，具有抗真菌活性的挥发性有机化合物（VOCs）的细菌合成表明，. 和 分别受到87.45%和77.24%的抑制。同时，,, 和 的平均径向生长抑制率分别为52.79%、40.82%和55.40%。抑制率最低的是 ，为10.64%。通过顶空固相微萃取结合气相色谱 - 质谱联用（HS - SPME - GC - MS）对VOCs进行表征，发现了65种潜在化合物。一些相对丰度较高的化合物被鉴定为酮类（22.47%），以3 - 羟基 - 2 - 丁酮（13.52%）为代表，以及醇类（23.5%），以乙醇（5.56%）和3 - 甲基 - 1 - 丁醇（4.83%）为代表。我们的研究首次揭示，与 种子相关的Cp1菌株具有潜在特性，表明它可作为一种有效的生物防治手段。
