从L.种子中分离出的CH05和CH13对真菌菌株的生物防治活性。

Biocontrol Activity of CH05 and CH13 Isolated from L. Seeds against Fungal Strains.

作者信息

Espinosa Bernal Merle Ariadna, Mena Navarro Mayra Paola, Arvizu Gómez Jackeline Lizzeta, Saldaña Carlos, Ramos López Miguel Ángel, Amaro Reyes Aldo, Escamilla García Monserrat, Pacheco Aguilar Juan Ramiro, Moreno Victor Pérez, Rodríguez Morales José Alberto, Álvarez Hidalgo Erika, Nuñez Ramírez Jorge, Hernández Flores José Luis, Campos Guillén Juan

机构信息

Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas S/N, Querétaro 76010, Querétaro, Mexico.

Secretaría de Investigación y Posgrado, Centro Nayarita de Innovación y Transferencia de Tecnología (CENITT), Universidad Autónoma de Nayarit, Tepic 63173, Nayarit, Mexico.

出版信息

Microorganisms. 2024 Sep 25;12(10):1943. doi: 10.3390/microorganisms12101943.

DOI:10.3390/microorganisms12101943

PMID:39458253

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11509363/

Abstract

In this study, seed-surface-associated bacteria from fresh fruits of spp. were analyzed to explore potential isolates for biocontrol of phytopathogenic fungal strains. A total of 76 bacterial isolates were obtained from three different species of chili pepper ( L., R. & P., and Jacq.), and two isolates were selected via mycelial growth inhibition assays based on their production of volatile organic compounds (VOCs) against six fungal strains. Genomic analysis identified these isolates as CH05, with a chromosome size of 3,687,823 bp and with 41.25% G+C, and CH13, with a chromosome size of 5,283,706 bp and with 35.24% G+C. Both bacterial strains showed high mycelial growth inhibition capacities against , sp., , and but lower inhibition capacities against and . VOC identification was carried out after 24 h of fermentation with 64 VOCs for CH05 and 53 VOCs for CH13. 2,5-Dimethyl pyrazine and acetoin had the highest relative abundance values in both bacterial strains. Our findings revealed that seed-surface-associated bacteria on spp. have the metabolic ability to produce VOCs for biocontrol of fungal strains and have the potential to be used in sustainable agriculture.

摘要

在本研究中，对辣椒属新鲜果实种子表面相关细菌进行了分析，以探索用于植物病原真菌菌株生物防治的潜在分离株。从三种不同的辣椒品种（辣椒、朝天椒和灯笼椒）中总共获得了76株细菌分离株，并通过基于挥发性有机化合物（VOCs）对六种真菌菌株的产生情况的菌丝生长抑制试验，选出了两株分离株。基因组分析将这些分离株鉴定为CH05，其染色体大小为3,687,823 bp，G+C含量为41.25%；以及CH13，其染色体大小为5,283,706 bp，G+C含量为35.24%。两种细菌菌株对尖孢镰刀菌、炭疽菌属、灰葡萄孢菌和立枯丝核菌均表现出较高的菌丝生长抑制能力，但对链格孢菌和黑曲霉的抑制能力较低。发酵24小时后进行VOC鉴定，CH05鉴定出64种VOCs，CH13鉴定出53种VOCs。2,5-二甲基吡嗪和乙偶姻在两种细菌菌株中的相对丰度值最高。我们的研究结果表明，辣椒属种子表面相关细菌具有产生VOCs用于真菌菌株生物防治的代谢能力，并且有潜力用于可持续农业。