Tsai Chia-Wen, Chang Wen-Shin, Yueh Te-Cheng, Wang Yun-Chi, Chin Yu-Ting, Yang Mei-Due, Hung Yi-Chih, Mong Mei-Chin, Yang Ya-Chen, Gu Jian, Bau Da-Tian
Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404333, Taiwan.
Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Cancers (Basel). 2023 Oct 10;15(20):4921. doi: 10.3390/cancers15204921.
Interleukin-8 (IL-8), a pro-inflammatory cytokine, is upregulated in CRC and plays an important role in its development and progression. Genetic variants in the gene may impact the risk of CRC by modulating IL-8 levels. Our primary objective was to investigate the role of genotypes in the development of CRC. To accomplish this, we employed the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to analyze the genotypes of rs4017, rs2227306, rs2227543, and rs1126647 in 362 CRC patients and 362 controls. Additionally, we evaluated the interactions between these genotypes and factors such as age, gender, smoking, alcohol consumption, and body mass index (BMI) status in relation to the risk of CRC. Furthermore, we utilized quantitative reverse transcription-PCR to measure the serum IL-8. The results demonstrated a significant difference in the distribution of rs4017 genotypes between the control and case groups ( for trend = 0.0059). Logistic regression analysis revealed that individuals with variant AA genotype had a 1.92-fold higher CRC risk (95% confidence interval [CI] = 1.28-2.89, = 0.0023). Moreover, carriers of the rs4017 AT + AA genotypes exhibited a significant association with CRC risk (odds ratio [OR] = 1.39, 95% CI = 1.02-1.91, = 0.0460). Additionally, individuals with rs4017 AA genotype displayed significantly elevated serum IL-8 compared to those with TT genotype at a 1.73-fold level ( < 0.0001), indicating a correlation between genotype and phenotype. In conclusion, the genotypes of rs4017, along with their associated expression levels, can potentially serve as predictive markers for the risk of CRC.
白细胞介素-8（IL-8）是一种促炎细胞因子，在结直肠癌（CRC）中表达上调，在其发生发展过程中起重要作用。该基因的遗传变异可能通过调节IL-8水平影响CRC风险。我们的主要目的是研究该基因型在CRC发生中的作用。为此，我们采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法分析了362例CRC患者和362例对照中rs4017、rs2227306、rs2227543和rs1126647的基因型。此外，我们评估了这些基因型与年龄、性别、吸烟、饮酒和体重指数（BMI）状态等因素之间的相互作用与CRC风险的关系。此外，我们利用定量逆转录PCR检测血清IL-8。结果显示，对照组和病例组之间rs4017基因型的分布存在显著差异（趋势检验P = 0.0059）。逻辑回归分析显示，携带变异AA基因型的个体患CRC的风险高1.92倍（95%置信区间[CI]=1.28 - 2.89，P = 0.0023）。此外，rs4017 AT + AA基因型携带者与CRC风险显著相关（优势比[OR]=1.39，95% CI = 1.02 - 1.91，P = 0.
