萝藦科娃儿藤属植物甾体生物碱及其抗炎活性研究

Steroidal Alkaloids from the Roots of Loes. with Their Anti-Inflammatory Activities.

机构信息

College of Science, Yunnan Agricultural University, Kunming 650201, China.

Key Laboratory of Pu'er Tea, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China.

出版信息

Molecules. 2023 Oct 16;28(20):7116. doi: 10.3390/molecules28207116.

The phytochemical investigation of Loes. roots resulted in the isolation and characterization of two novel, namely Mengtzeanines A (), Mengtzeanines B (), and eight known steroidal alkaloids (-). Their structural properties were assessed though extensive spectroscopic techniques. All constituents - were analyzed for suppression of NO formation in LPS-induced RAW264.7 macrophages. Among them, constituent (Verazine) showed inhibition against LPS-induced NO production (IC = 20.41 μM). Additionally, compound could inhibit the secretion of IL1β, IL6, and TNFα, and downregulate the productions of iNOS and COX2 in LPS-induced RAW264.7 macrophages. Further experiments revealed that exhibited a potent anti-inflammatory level in LPS-stimulated RAW264.7 macrophages via inhibiting NF-κB, and triggering of Keap1/Nrf2/HO-1 axis, implying that compound may be a promising candidate for treating inflammatory disorders.

洛氏根的植物化学研究导致了两种新型化合物的分离和鉴定，即 Mengtzeanines A ()、Mengtzeanines B ()和八种已知甾体生物碱 (-)。通过广泛的光谱技术评估了它们的结构特性。所有成分 - 都分析了它们对 LPS 诱导的 RAW264.7 巨噬细胞中 NO 形成的抑制作用。其中，成分（Verazine）显示出对 LPS 诱导的 NO 产生的抑制作用（IC = 20.41 μM）。此外，化合物可以抑制 LPS 诱导的 RAW264.7 巨噬细胞中 IL1β、IL6 和 TNFα 的分泌，并下调 LPS 诱导的 RAW264.7 巨噬细胞中 iNOS 和 COX2 的产生。进一步的实验表明，化合物通过抑制 NF-κB 和触发 Keap1/Nrf2/HO-1 轴，在 LPS 刺激的 RAW264.7 巨噬细胞中表现出强大的抗炎作用，这表明化合物可能是治疗炎症性疾病的有前途的候选药物。