College of Science, Yunnan Agricultural University, Kunming 650201, China.
Key Laboratory of Pu'er Tea, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China.
Molecules. 2023 Oct 16;28(20):7116. doi: 10.3390/molecules28207116.
The phytochemical investigation of Loes. roots resulted in the isolation and characterization of two novel, namely Mengtzeanines A (), Mengtzeanines B (), and eight known steroidal alkaloids (-). Their structural properties were assessed though extensive spectroscopic techniques. All constituents - were analyzed for suppression of NO formation in LPS-induced RAW264.7 macrophages. Among them, constituent (Verazine) showed inhibition against LPS-induced NO production (IC = 20.41 μM). Additionally, compound could inhibit the secretion of IL1β, IL6, and TNFα, and downregulate the productions of iNOS and COX2 in LPS-induced RAW264.7 macrophages. Further experiments revealed that exhibited a potent anti-inflammatory level in LPS-stimulated RAW264.7 macrophages via inhibiting NF-κB, and triggering of Keap1/Nrf2/HO-1 axis, implying that compound may be a promising candidate for treating inflammatory disorders.
洛氏根的植物化学研究导致了两种新型化合物的分离和鉴定，即 Mengtzeanines A ()、Mengtzeanines B ()和八种已知甾体生物碱 (-)。通过广泛的光谱技术评估了它们的结构特性。所有成分 - 都分析了它们对 LPS 诱导的 RAW264.7 巨噬细胞中 NO 形成的抑制作用。其中，成分 （Verazine）显示出对 LPS 诱导的 NO 产生的抑制作用（IC = 20.41 μM）。此外，化合物 可以抑制 LPS 诱导的 RAW264.7 巨噬细胞中 IL1β、IL6 和 TNFα 的分泌，并下调 LPS 诱导的 RAW264.7 巨噬细胞中 iNOS 和 COX2 的产生。进一步的实验表明，化合物 通过抑制 NF-κB 和触发 Keap1/Nrf2/HO-1 轴，在 LPS 刺激的 RAW264.7 巨噬细胞中表现出强大的抗炎作用，这表明化合物 可能是治疗炎症性疾病的有前途的候选药物。
