Department of Pharmacy, University of Patras, 265 04 Patras, Greece.
Foundation for Research and Technology, Institute of Chemical Engineering and High Temperatures, FORTH/ICE-HT, 265 04 Patras, Greece.
Molecules. 2023 Oct 20;28(20):7196. doi: 10.3390/molecules28207196.
Raman microspectroscopy and X-ray microcomputed tomography (micro-CT) were used for assessment of the quality of the femur and tibia bones in apolipoprotein-deficient mice compared to control littermates. The cortical and trabecular bone was investigated separately. Raman spectra revealed no differences in the bioapatite-to-collagenous matrix ratio of the cortical bone. The quantities of calcium and collagen, which were measured using atomic absorption spectrometry and thermogravimetric analysis, respectively, were also found to be equal in the two groups. Density and morphometric parameters, which were measured using micro-CT, verified the cortical mineral stability. Bone quality indices were measured using Raman spectra. A decreased collagen crosslink (trivalent-to-divalent) ratio revealed delayed maturation of the collagen network. Such a decrease has been reported in the literature to be connected to decreased bone strength. For the trabecular bone, micro-CT revealed severe osteoporosis in the knock-out group, which was evident from a decreased mineral density, trabecular thickness and increased bone surface/volume ratio. The trabecular bone was not accessible for Raman spectroscopy. According to these results, the cortical and trabecular femur bone is expected to exhibit proneness to fracturing, each for a different reason. A combination of the two techniques was regarded as necessary for an overall assessment of bone quality.
拉曼微光谱分析和 X 射线微计算机断层扫描（micro-CT）用于评估载脂蛋白缺陷型小鼠与对照同窝仔鼠的股骨和胫骨质量。分别对皮质骨和松质骨进行了研究。拉曼光谱显示皮质骨生物磷灰石与胶原基质的比值没有差异。使用原子吸收光谱法和热重分析法分别测量的钙和胶原蛋白的含量在两组中也相等。使用 micro-CT 测量的密度和形态计量参数证实了皮质矿化的稳定性。使用拉曼光谱测量骨质量指数。三价-二价胶原交联比的降低表明胶原网络的成熟延迟。文献报道，这种减少与骨强度降低有关。对于松质骨，micro-CT 显示敲除组存在严重的骨质疏松症，表现为骨矿物质密度降低、骨小梁厚度降低和骨表面积/体积比增加。松质骨无法进行拉曼光谱分析。根据这些结果，预计股骨的皮质骨和松质骨由于不同的原因而容易发生骨折。两种技术的结合被认为是进行骨质量全面评估的必要条件。
