Department of Neurodegenerative Diseases Research Group, Korea Brain Research Institute, 61, Cheomdan ro, Dong gu, Daegu 41062, Republic of Korea.
Laboratory Animal Center, Division of Research Strategy, Korea Brain Research Institute, 61, Cheomdan ro, Dong gu, Daegu 41062, Republic of Korea.
Int J Mol Sci. 2023 Oct 11;24(20):15064. doi: 10.3390/ijms242015064.
Alzheimer's disease (AD) is characterized by the accumulation of amyloid β (Aβ) plaques in the brain, leading to cognitive impairment and other clinical symptoms. The 5XFAD mouse model is commonly used in AD research because it expresses five human transgenes that result in the accumulation of Aβ plaques and cognitive decline at a relatively early age. Behavioral experiments are frequently conducted using this model; however, the effect size has not yet been reported. In this study, we examined basic cognition and locomotion in 5XFAD mice with a C57BL6/J background (5XFAD-J) at 6 months of age, a period in which impairments of cognitive function and locomotion are commonly observed. We analyzed the effect sizes of cognitive and locomotive experiments in the 5XFAD mice compared with those in the wild-type mice. Our results suggest that for long-term memory analysis, the novel object recognition test ( = 0.013, effect size 1.24) required a sample size of at least 12 to obtain meaningful results. Moreover, analysis of general locomotion over total distance with the Laboratory Animal Behavior Observation, Registration and Analysis System (LABORAS) test during the dark phase ( = 0.007, effect size -1.37) needed a sample size of 10 for a statistical power (1-β) of 0.8. In conclusion, we can conduct more ethical and scientifically rigorous animal experiments using 5XFAD mice based on the effect and sample sizes suggested in this study.
阿尔茨海默病（AD）的特征是大脑中淀粉样β（Aβ）斑块的积累，导致认知障碍和其他临床症状。5XFAD 小鼠模型常用于 AD 研究，因为它表达了五个人类转基因，导致 Aβ斑块的积累和认知能力下降在相对较早的年龄。该模型经常用于行为实验；然而，尚未报道效应大小。在这项研究中，我们检查了具有 C57BL6/J 背景的 5XFAD 小鼠（5XFAD-J）的基本认知和运动能力，在这个年龄，认知功能和运动能力的损伤通常是观察到的。我们分析了 5XFAD 小鼠与野生型小鼠相比，在认知和运动实验中的效应大小。我们的研究结果表明，对于长期记忆分析，新物体识别测试（ = 0.013，效应大小 1.24）需要至少 12 个样本才能获得有意义的结果。此外，使用实验室动物行为观察、登记和分析系统（LABORAS）在暗期分析总距离的一般运动（ = 0.007，效应大小-1.37）需要 10 个样本才能达到统计功效（1-β）为 0.8。总之，我们可以根据本研究中建议的效应和样本大小，使用 5XFAD 小鼠进行更符合伦理和科学严格的动物实验。
