CHA Fertility Center Seoul Station, Seoul 04637, Republic of Korea.
Department of Biomedical Sciences, CHA University, Pocheon 11160, Republic of Korea.
Int J Mol Sci. 2023 Oct 13;24(20):15158. doi: 10.3390/ijms242015158.
Ovarian aging is a major obstacle in assisted reproductive medicine because it leads to ovarian dysfunction in women of advanced age. Currently, there are no effective treatments to cure age-related ovarian dysfunction. In this study, we investigated the effect of MIT-001 on the function of aged ovaries. Young and old mice were utilized in this study. MIT-001 was intraperitoneally administered, and the number of follicles and oocytes was analyzed. Each group was then retrieved for RNA and protein isolation. Total RNA was subjected to mRNA next-generation sequencing. Protein extracts from ovarian lysates were used to evaluate various cytokine levels in the ovaries. MIT-001 enhanced follicles and the number of oocytes were compared with non-treated old mice. MIT-001 downregulated immune response-related transcripts and cytokines in the ovaries of old mice. MIT-001 modulates the immune complex responsible for generating inflammatory signals and has the potential to restore the function of old ovaries and improve female fertility.
卵巢衰老（Ovarian aging）是辅助生殖医学的主要障碍，因为它会导致高龄女性的卵巢功能障碍。目前，尚无有效的治疗方法来治愈与年龄相关的卵巢功能障碍。在这项研究中，我们研究了 MIT-001 对衰老卵巢功能的影响。本研究使用了年轻和老年小鼠。通过腹腔内给药 MIT-001，并分析卵泡和卵母细胞的数量。然后对每组进行 RNA 和蛋白质分离。对总 RNA 进行 mRNA 下一代测序。从卵巢裂解物中提取蛋白提取物，以评估卵巢中各种细胞因子的水平。MIT-001 增强了卵泡和卵母细胞的数量，与未治疗的老年小鼠相比。MIT-001 下调了老年小鼠卵巢中与免疫反应相关的转录本和细胞因子。MIT-001 调节了产生炎症信号的免疫复合物，有可能恢复老年卵巢的功能并提高女性生育能力。
