Laboratory of Proteomics, Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia.
Glasgow Polyomics, Wolfson Wohl Cancer Research Centre, University of Glasgow, Glasgow G61 1QH, UK.
Int J Mol Sci. 2023 Oct 14;24(20):15182. doi: 10.3390/ijms242015182.
Dilated cardiomyopathy is one of the important diseases in dogs and humans. The second most common cause of heart failure in dogs is idiopathic dilated cardiomyopathy (iDCM), which results in heart failure or sudden cardiac death due to arrhythmia. This study aimed to determine changes in the plasma metabolome of dogs with iDCM compared to healthy dogs. For that purpose, a multiplatform mass-spectrometry-based approach was used. In this study, we included two groups of dogs: 12 dogs with iDCM and 8 healthy dogs. A total of 272 metabolites were detected in the plasma samples of dogs by combining three approaches but four MS-based platforms (GC-MS, LC-MS (untargeted), LC-MS (targeted), and FIA-MS (targeted) methods). Our findings demonstrated changes in the canine plasma metabolome involved in the development of iDCM, including the different concentrations of amino acids, biogenic amines, acylcarnitines, triglycerides and diglycerides, sphingomyelins, and organic acids. The results of this study will enable the detection and monitoring of pathophysiological mechanisms involved in the development of iDCM in the future.
扩张型心肌病是犬和人类的重要疾病之一。犬心力衰竭的第二大常见病因是特发性扩张型心肌病（iDCM），由于心律失常而导致心力衰竭或心源性猝死。本研究旨在确定与健康犬相比，iDCM 犬的血浆代谢组发生的变化。为此，采用了多平台基于质谱的方法。在这项研究中，我们纳入了两组犬：12 只患有 iDCM 的犬和 8 只健康犬。通过结合三种方法（GC-MS、LC-MS（非靶向）、LC-MS（靶向）和 FIA-MS（靶向）方法），在犬的血浆样本中检测到了 272 种代谢物。我们的研究结果表明，与 iDCM 发展相关的犬血浆代谢组发生了变化，包括不同浓度的氨基酸、生物胺、酰基辅酶 A、甘油三酯和甘油二酯、鞘磷脂和有机酸。这项研究的结果将能够在未来检测和监测与 iDCM 发展相关的病理生理机制。
