Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya 572024, China.
Int J Mol Sci. 2023 Oct 21;24(20):15424. doi: 10.3390/ijms242015424.
The original 'Green Revolution' genes are associated with gibberellin deficiency. However, in some species, mutations in these genes cause pleiotropic phenotypes, preventing their application in dwarf breeding. The development of novel genotypes with reduced plant height will resolve this problem. In a previous study, we obtained two dwarf lines, L28 and L30, by introducing the (Maxim. ex Kom.) Cheng f. () into the upland cotton variety R15. We found that () was downregulated in L28 and L30, which suggested that this gene may have contributed to the dwarf phenotype of L28 and L30. Here, we tested this hypothesis by silencing expression in R15 and found that decreased expression resulted in a dwarf phenotype. Interestingly, we found that repressing expression in L28 and L30 partly recovered the expression of . Thus, expression presented a negative relationship with expression in L28 and L30. Moreover, yeast one-hybrid and dual-luciferase assays suggest that AmCBF1 negatively regulates expression by directly binding to C-repeat/dehydration-responsive (CRT/DRE) elements in the promoter, potentially explaining the dwarf phenotypes of L28 and L30. This study elucidates the regulation of expression by AmCBF1 and suggests that may be a new target gene for breeding dwarf and compact cultivars.
最初的“绿色革命”基因与赤霉素缺乏有关。然而，在某些物种中，这些基因的突变会导致多效表型，从而阻止它们在矮化育种中的应用。开发新型的矮化基因型将解决这个问题。在之前的一项研究中，我们通过将（Maxim. ex Kom.）Cheng f. （）导入陆地棉品种 R15 获得了两个矮秆系 L28 和 L30。我们发现 L28 和 L30 中下调，这表明该基因可能导致了 L28 和 L30 的矮秆表型。在这里，我们通过沉默 R15 中的表达来验证这个假设，发现表达下调导致矮秆表型。有趣的是，我们发现抑制 L28 和 L30 中的表达部分恢复了的表达。因此，在 L28 和 L30 中，表达与表达呈负相关。此外，酵母单杂交和双荧光素酶报告基因 assays 表明，AmCBF1 通过直接结合在的启动子中的 C-重复/脱水应答（CRT/DRE）元件负调控表达，这可能解释了 L28 和 L30 的矮秆表型。本研究阐明了 AmCBF1 对表达的调控，并表明可能是培育矮化紧凑品种的一个新的靶基因。
