Daskalova Albena, Sezanova Kostadinka, Angelova Liliya, Paunova-Krasteva Tsvetelina, Gergulova Rumiana, Kovacheva Daniela, Rabadjieva Diana
Institute of Electronics, Bulgarian Academy of Sciences, 1784 Sofia, Bulgaria.
Institute of General and Inorganic Chemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.
Materials (Basel). 2023 Oct 10;16(20):6626. doi: 10.3390/ma16206626.
Bacterial infection is one of the most common and harmful medical issues following the implantation of materials and devices in the body leading to antibiotic resistance of diverse bacterial strains. In this work, a novel approach is presented combining adopted laser-based patterning method in addition to doping (Mg and Zn) metal ions to prepare calcium phosphate ceramic substrate, applicable in medicine, with enhanced surface antimicrobial characteristics. The preparation of tablets containing Mg (8.44 mol%) and Zn (2.63 mol%) β-tricalcium phosphate involved biomimetic precipitation of amorphous calcium phosphate in media of simulated body fluid enriched with Mg and Zn ions as well as the presence of valine as an organic additive, followed by step-wise calcination up to 1000 °C. The results from laser processing showed formation of deeper patterns with increased surface roughness (from 4.9 µm to 9.4 µm) as laser power and velocity increase, keeping constant the hatch sizes of 50 µm. The textured surfaces consist of peaks and valleys arrangement that change the morphology of cells and decrease of cell viability. Our study reveals the possibilities of the application of ultra-short laser radiation as a potential alternative therapy for controlling the antimicrobial effect of the ceramic surface.
细菌感染是体内植入材料和装置后最常见且有害的医学问题之一，会导致多种细菌菌株产生抗生素耐药性。在这项工作中，提出了一种新颖的方法，除了对金属离子（镁和锌）进行掺杂外，还结合采用基于激光的图案化方法来制备适用于医学的具有增强表面抗菌特性的磷酸钙陶瓷基板。含有镁（8.44摩尔%）和锌（2.63摩尔%）的β-磷酸三钙片剂的制备过程包括在富含镁和锌离子的模拟体液介质以及缬氨酸作为有机添加剂存在的情况下，对无定形磷酸钙进行仿生沉淀，随后逐步煅烧至1000°C。激光加工结果表明，随着激光功率和速度的增加，在保持50μm的孵化尺寸不变的情况下，会形成更深的图案且表面粗糙度增加（从4.9μm增加到9.4μm）。纹理化表面由峰谷排列组成，这种排列会改变细胞形态并降低细胞活力。我们的研究揭示了应用超短激光辐射作为控制陶瓷表面抗菌效果的潜在替代疗法的可能性。
