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用于发育模型中承重骨再生的矿化胶原蛋白/聚乳酸复合支架

Mineralized Collagen/Polylactic Acid Composite Scaffolds for Load-Bearing Bone Regeneration in a Developmental Model.

作者信息

Zhu Wenbo, Li Wenjing, Yao Mengxuan, Wang Yan, Zhang Wei, Li Chao, Wang Xiumei, Chen Wei, Lv Hongzhi

机构信息

Department of Orthopaedic Surgery, Hebei Medical University Third Hospital, No. 139 Ziqiang Road, Shijiazhuang 050051, China.

Key Laboratory of Biomechanics of Hebei Province, Orthopaedic Research Institution of Hebei Province, No. 139 Ziqiang Road, Shijiazhuang 050051, China.

出版信息

Polymers (Basel). 2023 Oct 23;15(20):4194. doi: 10.3390/polym15204194.

DOI:10.3390/polym15204194
PMID:37896438
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10610794/
Abstract

Repairing load-bearing bone defects in children remains a big clinical challenge. Mineralized collagen (MC) can effectively simulate natural bone composition and hierarchical structure and has a good biocompatibility and bone conductivity. Polylactic acid (PLA) is regarded as a gold material because of its mechanical properties and degradability. In this study, we prepare MC/PLA composite scaffolds via in situ mineralization and freeze-drying. Cell, characterization, and animal experiments compare and evaluate the biomimetic properties and repair effects of the MC/PLA scaffolds. Phalloidin and DAPI staining results show that the MC/PLA scaffolds are not cytotoxic. CCK-8 and scratch experiments prove that the scaffolds are superior to MC and hydroxyapatite (HA)/PLA scaffolds in promoting cell proliferation and migration. The surface and interior of the MC/PLA scaffolds exhibit rich interconnected pore structures with a porosity of ≥70%. The XRD patterns are typical HA waveforms. X-ray, micro-CT, and H&E staining reveal that the defect boundary disappears, new bone tissue grows into MC/PLA scaffolds in a large area, and the scaffolds are degraded after six months of implantation. The MC/PLA composite scaffold has a pore structure and composition similar to cancellous bone, with a good biocompatibility and bone regeneration ability.

摘要

修复儿童承重骨缺损仍然是一项重大的临床挑战。矿化胶原蛋白（MC）能够有效模拟天然骨的组成和层级结构，并且具有良好的生物相容性和骨传导性。聚乳酸（PLA）因其机械性能和可降解性而被视为一种优质材料。在本研究中，我们通过原位矿化和冷冻干燥制备了MC/PLA复合支架。细胞、表征及动物实验对MC/PLA支架的仿生性能和修复效果进行了比较和评估。鬼笔环肽和DAPI染色结果表明MC/PLA支架无细胞毒性。CCK-8和划痕实验证明，该支架在促进细胞增殖和迁移方面优于MC和羟基磷灰石（HA）/PLA支架。MC/PLA支架的表面和内部呈现出丰富的相互连通的孔隙结构，孔隙率≥70%。XRD图谱为典型的HA波形。X射线、显微CT和苏木精-伊红染色显示，缺损边界消失，大面积新骨组织长入MC/PLA支架，植入六个月后支架发生降解。MC/PLA复合支架具有与松质骨相似的孔隙结构和组成，具有良好的生物相容性和骨再生能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/c6ef8bfba0d4/polymers-15-04194-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/6efdb76a2541/polymers-15-04194-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/57d718f865ef/polymers-15-04194-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/a3d7bd14934c/polymers-15-04194-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/4017c04db1d2/polymers-15-04194-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/42f483e9a9d3/polymers-15-04194-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/46b385e60b58/polymers-15-04194-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/b6cb6311afa7/polymers-15-04194-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/a286b03b6226/polymers-15-04194-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/c6ef8bfba0d4/polymers-15-04194-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/6efdb76a2541/polymers-15-04194-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/57d718f865ef/polymers-15-04194-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/a3d7bd14934c/polymers-15-04194-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/4017c04db1d2/polymers-15-04194-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/42f483e9a9d3/polymers-15-04194-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/46b385e60b58/polymers-15-04194-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/b6cb6311afa7/polymers-15-04194-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/a286b03b6226/polymers-15-04194-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/10610794/c6ef8bfba0d4/polymers-15-04194-g009.jpg

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