Dept. of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk, 304022, Rajasthan, India.
J Fluoresc. 2024 Nov;34(6):2725-2735. doi: 10.1007/s10895-023-03480-0. Epub 2023 Oct 28.
Mitochondria transplantation has emerged as a successful therapeutic modality to treat several degenerative diseases. However, the biodistribution of transplanted mitochondria has not been well studied. We investigated the ex-vivo systemic biodistribution and therapeutic efficacy of intravenously transplanted graphene quantum dots (GQDs) conjugated to isolated mitochondria (Mt-GQDs) in diabetic rat tissues. The results revealed that Mt-GQDs facilitate the tracking of transplanted mitochondria without affecting their therapeutic efficacy. It is compelling to note that Mt-GQDs and isolated mitochondria show comparable therapeutic efficacies in decreasing blood glucose levels, oxidative stress, inflammatory gene expressions, and restoration of different mitochondrial functions in pancreatic tissues of diabetic rats. In addition, histological section examination under a fluorescence microscope demonstrated the localization of Mt-GQDs in multiple tissues of diabetic rats. In conclusion, this study indicates that Mt-GQDs provide an effective mitochondrial transplantation tracking modality.
线粒体移植已成为治疗多种退行性疾病的成功治疗方法。然而，移植线粒体的生物分布尚未得到很好的研究。我们研究了静脉内移植的与分离的线粒体（Mt-GQDs）缀合的石墨烯量子点（GQDs）在糖尿病大鼠组织中的体内系统生物分布和治疗效果。结果表明，Mt-GQDs 促进了对移植线粒体的跟踪，而不影响其治疗效果。值得注意的是，Mt-GQDs 和分离的线粒体在降低血糖水平、氧化应激、炎症基因表达以及恢复糖尿病大鼠胰腺组织中不同的线粒体功能方面具有相当的治疗效果。此外，荧光显微镜下的组织切片检查显示 Mt-GQDs 在糖尿病大鼠的多种组织中定位。总之，这项研究表明 Mt-GQDs 提供了一种有效的线粒体移植跟踪方式。
