温度对Tn3元件易位频率的影响。
Effect of temperature on translocation frequency of the Tn3 element.
作者信息
Kretschmer P J, Cohen S N
出版信息
J Bacteriol. 1979 Aug;139(2):515-9. doi: 10.1128/jb.139.2.515-519.1979.
Abstract
The effect of temperature on the translocation frequency of the Tn3 element was investigated. The temperature optimum for translocation of Tn3 was in the range from 26 to 30 degrees C. At temperatures above 30 degrees C, the translocation frequency decreased rapidly and linearly; at 36 degrees C it was only 5% of the frequency observed at 30 degrees C. The duration and reversibility of the temperature effect were utilized to demonstrate a requirement for protein synthesis in the translocation process.
摘要
研究了温度对Tn3元件易位频率的影响。Tn3易位的最适温度范围为26至30摄氏度。在高于30摄氏度的温度下，易位频率迅速且呈线性下降；在36摄氏度时，其频率仅为30摄氏度时观察到的频率的5%。利用温度效应的持续时间和可逆性来证明易位过程中蛋白质合成的必要性。
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