细菌中的非染色体抗生素抗性。五、对表现出对育性温度敏感抑制的R因子突变体的分离与特性研究
Nonchromosomal antibiotic resistance in bacteria. V. Isolation and characterization of R factor mutants exhibiting temperature-sensitive repression of fertility.
作者信息
Silver R P, Cohen S N
出版信息
J Bacteriol. 1972 Jun;110(3):1082-8. doi: 10.1128/jb.110.3.1082-1088.1972.
Abstract
Several distinct types of derepressed R factor mutants have been isolated and identified following nitrosoguanidine mutagenesis. One class of mutants appears to synthesize a temperature-sensitive repressor substance, as shown by the effects of temperature on R factor transfer, on male-specific phage sensitivity, and on F factor and Hfr transfer. In addition, two classes of repressor-in-sensitive derepressed R factor mutants have been identified: although both inhibit F factor transfer at 42 and 32 C, one mutant (R6-5drd50) represses its own fertility at only the low temperature. These results imply the existance of a plasmid-specific component of the R factor repression system, in addition to a component which acts on both R and F factor fertility.
摘要
在亚硝基胍诱变后，已分离并鉴定出几种不同类型的去阻遏R因子突变体。一类突变体似乎合成了一种温度敏感的阻遏物质，这可从温度对R因子转移、对雄性特异性噬菌体敏感性以及对F因子和Hfr转移的影响中看出。此外，还鉴定出了两类对阻遏物不敏感的去阻遏R因子突变体：尽管两者在42℃和32℃时都抑制F因子转移，但一个突变体（R6 - 5drd50）仅在低温下抑制自身的育性。这些结果表明，除了一个作用于R和F因子育性的成分外，R因子阻遏系统还存在一个质粒特异性成分。
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